作者： Liu, An;Gong, Zhihua;Lin, Ling;Xu, Wei;Zhang, Tuo;...

期刊： Journal of Functional Foods,2020年64:103670 ISSN：1756-4646

通讯作者： Xiao, Wenjun

作者机构： [Xiao, Wenjun] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Xiao, Wenjun] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

关键词： l-theanine;l;Nutritional intervention;Glutamine metabolism;Enterotoxigenic Escherichia coli (E44813) stress;Exogenous l-glutamine;l;Synergistic regulation

摘要： L-theanine, a non-protein-derived amino acid, is widely used as a functional ingredient in foods. Here, we evaluated the in vivo effects of L-theanine on glutamine metabolism in healthy rats and in a stressed rat model, which was established by infecting rats with enterotoxigenic Escherichia coli (E44813). The results showed that L-theanine significantly enhanced the synthesis of glutamine and stabilized the intestinal tract in healthy rats. In E44813-stressed rats, L-theanine could replace L-glutamine, thereby alleviating intestinal stress by reducing the expression of inflammatory factors, enhancing the activities of antioxidant enzymes, and regulating glutamine metabolism by modulating its synthesis. Treatment with a combination of L-theanine and L-glutamine was superior to treatment with L-glutamine alone in enhancing the activity of antioxidant enzymes, reducing the expression of inflammatory factors, and increasing the intestinal villi height and crypt depth. © 2019 Elsevier Ltd

语种： 英文

作者： Zeng, Li;Lin, Ling;Peng, Yingqi;Yuan, Dongyin;Zhang, Sheng;...

期刊： Experimental Gerontology,2020年131:110823 ISSN：0531-5565

通讯作者： Gong, Zhihua;Xiao, Wenjun

作者机构： [Yuan, Dongyin; Zeng, Li; Gong, Zhihua; Gong, ZH; Xiao, WJ; Xiao, Wenjun; Peng, Yingqi; Zhang, Sheng; Lin, Ling] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.;[Zeng, Li] Shaoyang Univ, Sch Pharm, Shaoyang 422000, Peoples R China.;[Yuan, Dongyin; Zeng, Li; Gong, Zhihua; Xiao, Wenjun; Peng, Yingqi; Zhang, Sheng; Lin, Ling] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Gong, ZH; Xiao, WJ] H;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.

关键词： AGEs;Inflammatory response;Liver aging;Oxidative stress;l-Theanine

摘要： Redox and inflammation imbalances are associated with increased levels of advanced glycation end products (AGEs), leading to the degeneration of body function. L-Theanine, derived from tea, reportedly inhibits AGE formation in vitro. We investigated the effects on AGE content, oxidative stress, and inflammatory factors in D-galactose-induced aging rats for prevention and treatment of age-related liver dysfunction. L-Theanine increased activities of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase, thus enhancing total antioxidant capacity, and decreasing malondialdehyde and nitric oxide synthase levels in serum and liver. Levels of the pro-inflammatory factors, interleukin (IL)-1 beta, tumour necrosis factor-alpha, and IL-6 were decreased in serum and liver, whereas those of anti-inflammatory factors, IL-4 and IL-10, were increased in the serum. Further, L-theanine inhibited AGE production and decreased the levels of the liver function markers, alanine aminotransaminase and aspartate aminotransferase. It also significantly increased the mRNA expression levels of FoxO1 and downregulated NF-kappa B(p65) but suppressed the phosphorylation of both FOXO1 and NF-kappa B (p65). Moreover, L-theanine effectively attenuated n-galactose-induced oedema and vacuole formation, thus protecting the liver. Overall, L-theanine reversed the D-galactose-induced imbalance in oxidative stress and inflammatory responses, reduced AGEs content in aging rats, maintained homeostasis in the body, and ameliorated liver aging.

语种： 英文

作者： Li, Penghui;Liu, Ailing;Xiong, Wei;Lin, Haiyan;Xiao, Wenjun;...

期刊： Critical Reviews in Food Science and Nutrition,2020年60(3):515-528 ISSN：1040-8398

通讯作者： Zhang, Sheng

作者机构： [Liu, Zhonghua; Lin, Haiyan; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Lin, Haiyan; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan] Hunan Agr Univ, Collaborat Innovat Ctr Utilisat Funct Ingredients, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha, Hunan, Peoples R China.;[Liu, Ailing] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Chinese Minist Hlth, Key Lab Carcinogenesis, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Chinese Minist Hlth, Canc Res Inst, Key Lab Carcinogenesis & Canc Invas, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, Sheng] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

关键词： Catechin;metabolism;mitochondrial;muscle atrophy;skeletal muscle

摘要： Muscle-related disorders, such as sarcopenia and cachexia, caused by aging and chronic diseases can lead to the loss of muscle mass and strength to different degrees, severely affecting human health. Globally, tea is one of the three most popular beverages, and its major active ingredient catechins have been reported to delay muscular atrophy and enhance movement. However, currently, there is no systematic review to elaborate its roles and the associated mechanisms. This article reviews the (1) functions and mechanisms of catechins in the differentiation of myogenic stem cells, biogenesis of mitochondria, synthesis and degradation of proteins, regulation of glucose level, and metabolism of lipids in muscle cells; and (2) effect of catechins on the blood vessels, bones, and nerves that are closely related to the skeletal muscles. Catechins could prevent, mitigate, delay, and even treat muscle-related disorders caused by aging and diseases. © 2019, © 2019 Taylor & Francis Group, LLC.

语种： 英文

作者： 李银花;李娟;刘仲华;李适;肖文军

期刊： 茶叶通讯,2019年46(2):253-256 ISSN：1009-525X

作者机构： 湖南农业大学 园艺园林学院 茶学系,茶学教育部重点实验室,湖南 长沙 410128;[李银花; 刘仲华; 李娟; 李适; 肖文军] 湖南农业大学

关键词： 教学;科研;互动;创新人才;培养模式

摘要： 本科教育是现代高等教育的重要基础。茶学是中国的特色专业,随着茶产业的快速发展,社会迫切需要大量茶学创新型技术人才。本文以湖南农业大学茶学本科专业为例,分析了茶学专业创新人才培养的现状和存在的问题,探索基于教学科研互动的茶学创新人才培养模式,旨在提高茶学专业人才培养质量,为进一步深化茶学专业创新教育改革提供依据。

语种： 中文

作者： Gong, Zhihua;Lin, Ling;Liu, Zunying;Zhang, Sheng;Liu, An;...

期刊： Journal of Functional Foods,2019年54:32-40 ISSN：1756-4646

通讯作者： Deng, Yanli;Xiao, Wenjun

作者机构： [Gong, Zhihua; Liu, Zunying; Liu, An; Xiao, Wenjun; Zhang, Sheng; Deng, Yanli; Xiao, WJ; Liu, Qiuling; Lin, Ling; Chen, Ling] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Gong, Zhihua; Liu, Zunying; Liu, An; Xiao, Wenjun; Zhang, Sheng; Deng, Yanli; Xiao, WJ; Liu, Qiuling; Lin, Ling; Chen, Ling] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.;[Gong, Zhihua; Liu, Zunying; Liu, An; Xiao, Wenjun; Zhang, Sheng; Deng, Yanli; Xiao, WJ; Liu, Qiuling; Lin, Ling; Chen, Ling] Hunan Agr Univ, Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410128, Hunan, Peoples R China.;[Deng, Yanli] Guizhou Univ, Tea Coll, Guiyang 550025, Guizhou, Peoples R China.

通讯机构： [Deng, YL; Xiao, WJ] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410128, Hunan, Peoples R China.

关键词： L-Theanine;Immune stress;Innate immunity;Nucleotide-binding oligomerization domain-like receptor;Immune mechanism

摘要： L-Theanine (LTA), a non-protein-derived amino acid, is widely used as a functional ingredient. Here, we established an immune-stressed mouse model by infecting mice with enterotoxigenic Escherichia coli (ETEC) to study the immunomodulatory effects of LTA with respect to nucleotide-binding oligomerization domain (NOD)-like receptor signaling in vivo. Compared to ETEC control group, LTA alleviated ileal tissue lesions, significantly attenuated interleukin-1 beta (IL-1β) and tumor necrosis alpha (TNFα) overexpression, which was consistent with their protein levels. Additionally, LTA inhibited the ETEC-induced overexpression of NOD2 and receptor-interacting protein 2 (RIP2) proteins and mRNAs, TGE-beta-activated kinase 1 (TAK1) and proteasome subunit alpha type-7 (PSMA7) mRNAs, and partially decreased nuclear factor-κB (NF-κB) p65 protein levels. Furthermore, it suppressed c-Jun NH2-terminal kinase 1/2 (JNK1/2), extracellular signal-regulated kinase 1/2 (ERK1/2), and p38 mitogen-activated protein kinase (p38) phosphorylation. Thus, we demonstrated that LTA regulates the innate immunity of ETEC-induced immune-stressed mice via NOD1/2-NF-κB and NOD1/2-MAPK pathways. © 2019

语种： 英文

作者： 张拓;刘林峰;林玲;周阳;肖文军;...

期刊： 茶叶通讯,2019年46(2):192-200 ISSN：1009-525X

作者机构： 湖南农业大学 茶学教育部重点实验室,国家植物功能成分利用工程技术研究中心,湖南 长沙 410128;湖南农业大学 湖南省植物功能成分利用协同创新中心,湖南 长沙 410128;[张拓; 林玲; 龚志华; 肖文军; 刘林峰; 周阳] 湖南农业大学

关键词： 茶树紫色芽叶;花色苷;高速逆流色谱;半制备型高效液相色谱

摘要： 花色苷是植物中一类具有重要生物活性的次生代谢产物,因其含量较低,使得分离制备花色苷高纯品难度较大.本研究以茶树紫芽为实验材料,以花色苷得率与纯度为考察指标,优化筛选了应用高速逆流色谱结合半制备型液相色谱分离纯化花色苷高纯度的方法.结果表明,茶样经提取、脱脂、阳离子交换树脂分离等前处理后,总花色苷得率28.18 mg/g,纯度为20.38％;以高速逆流色谱分离的最优溶剂体系乙酸乙酯:正丁醇:乙腈:0.1％ 三氟乙酸水溶液=8:35:13:60(v/v/v/v),除去部分杂质后,总花色苷得率达到160.59 mg/g,纯度为43.64％,再结合半制备型液相色谱分离出5种花色苷组分,纯度最高达90.61％,其优化色谱条件为C18制备柱、波长280 nm、柱温30℃、流动相1％醋酸水溶液(A)-乙腈(B)、流速5 mL/min、进样量1 mL、梯度洗脱(0～55 min,10％～40％B).研究表明高速逆流色谱结合半制备型液相色谱可有效地分离纯化茶树紫芽花色苷及其组分.

语种： 中文

作者： Tan, Lin;Gu, Songsong;Li, Shi;Ren, Zuohua;Deng, Ye;...

期刊： Sustainability,2019年11(16):4428 ISSN：2071-1050

通讯作者： Hu, Qiulong

作者机构： [Ren, Zuohua; Tan, Lin; Hu, Qiulong; Gu, Songsong] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Li, Shi; Gong, Zhihua; Liu, Zhonghua; Xiao, Wenjun] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Deng, Ye] Chinese Acad Sci, Key Lab Environm Biotechnol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China.

通讯机构： [Hu, Qiulong] H;Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.

关键词： long-term field experiments;high-throughput sequencing;management practice;microbial diversity;microbial community;network analysis

摘要： Soil microorganisms play important roles in the plant health and agricultural production. However, little is known about the complex responses of microbial communities and interaction networks to different agricultural management practices in tea plantation soils. In the present study, Illumina Miseq high-throughput sequencing technology and molecular ecological network (MEN) analysis were used to investigate the soil microbial diversity, community structure and composition, interaction networks of organic tea plantation (OTP), non-polluted tea plantation (NPTP) and conventional tea plantation (CTP). Alpha-diversity indices, Chao1 and richness, of OTP soil were significantly higher than those of NPTP and CTP soils. The beta-diversity analysis showed there were significant differences among bacterial community structures of OTP, NPTP and CTP soils. Composition analysis showed that Proteobacteria, Acidobacteria and Chloroflexi were the most dominant bacteria in all tea plantation soil samples under different management practices, and the beneficial community compositions of OTP soil were significantly different from NPTP and CTP soils at the phylum and genus levels. Canonical correspondence analysis (CCA) and mantel test revealed that TOC and NO3-N contents as well as pH values were the key soil factors to affect the bacterial community structures of tea plantation soils. Furthermore, network analysis showed that the network of OTP soil possessed more functionally interrelated microbial modules than NPTP and CTP soils, indicating that OTP soil possessed the higher ecosystem multi-functionality. These results provided the theoretical basis and reference for improving soil microbial diversity and enhancing community multi-functionality in tea plantation soil ecosystems through effective agricultural management practices. © 2019 by the authors.

语种： 英文

作者： 张娇;梁壮仙;张拓;李宗琼;朱静静;...

期刊： 食品科学,2019年40(16):200-205 ISSN：1002-6630

作者机构： 湖南农业大学 茶学教育部重点实验室,国家植物功能成分利用工程技术研究中心,湖南 长沙 410128;湖南省植物功能成分利用协同创新中心,湖南 长沙 410128;[张拓; 梁壮仙; 朱静静; 张娇; 李宗琼; 肖文军; 张鹏程] 湖南农业大学

关键词： 茶叶加工;黄茶;滋味品质;香气品质;动态变化

摘要： 以茶树品种碧香早夏季一芽一叶鲜叶为原料,按照摊放、杀青、揉捻、闷黄、干燥的传统黄茶加工工艺,将其加工成黄茶,通过对其中11个有代表性的工艺点取样,并进行茶多酚、儿茶素、氨基酸、水浸出物、可溶性糖、咖啡碱、黄酮含量检测,以及感官审评和香气品质成分分析,探讨黄茶加工过程中主要品质成分的动态变化。结果表明,在黄茶加工过程中,滋味物质茶多酚、儿茶素总量、水浸出物、可溶性糖、咖啡碱以及黄酮含量均在整体上逐渐降低,其足干茶样含量相对茶鲜叶的降幅分别为30.12%、24.08%、10.97%、61.34%、33.20%、25.55%,而游离氨基酸含量则有所增加,其足干茶样含量相对茶鲜叶的增幅为19.46%,其中茶多酚、咖啡碱、黄酮以及氨基酸含量在摊放过程中有所上升;香气物质中,足干茶样的醇类、酮类、烯烃类、酯类化合物的相对含量相较于茶鲜叶分别减少了17.18%、5.51%、5.77%、2.10%,而醛类、酚类化合物的相对含量则分别增加了35.35%、4.06%;从加工工艺看,黄茶滋味品质主要受摊放与闷黄工序的影响,摊放过程中氨基酸含量随着摊放时间的延长而增加,而闷黄过程可使酯型儿茶素转化为简单儿茶素,并在此工艺中产生茶黄素,是黄茶醇鲜爽滋味及"黄汤黄叶"品质形成的主要因素,同时闷黄时间为8 h时,滋味品质较好;香气品质则主要受摊放、闷黄和干燥工序影响。

语种： 中文

作者： Li, Penghui;Liu, Ailing;Li, Yinhua;Yuan, Bin;Xiao, Wenjun;...

期刊： Molecules,2019年24(2):24020323-1-24020323-8 ISSN：1420-3049

通讯作者： Zhang, Sheng;Lin, Haiyan

作者机构： [Li, Yinhua; Liu, Zhonghua; Xiao, Wenjun; Lin, Haiyan; Zhang, Sheng; Lin, HY; Li, Penghui] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.;[Li, Yinhua; Liu, Zhonghua; Xiao, Wenjun; Lin, Haiyan; Zhang, Sheng; Lin, HY; Li, Penghui] Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Liu, Ailing; Yuan, Bin] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Zhang, S; Lin, HY] H;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.

关键词： abietane derivative;carnosic acid;carnosol;cinnamic acid derivative;depside;oleanolic acid;rosmarinic acid;triterpene;ursolic acid;calibration;chemistry;high performance liquid chromatography;limit of detection;procedures;radiation scattering;reproducibility;validation study;Calibration;Chromatography, High Pressure Liquid;Cinnamates;Depsides;Diterpenes, Abietane;Limit of Detection;Oleanolic Acid;Reproducibility of Results;Scattering, Radiation;Triterpenes

摘要： The safety, efficacy and stability of natural antioxidants have been the focus of research in the food industry, with the aim of rapidly analyzing and controlling the quality of rosemary and its extracts, a novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous determination of rosmarinic acid, carnosol, carnosic acid, oleanolic acid and ursolic acid in rosemary. Chromatographic separation was conducted with gradient elution mode by using a Zorbax SB-C18 column (4.6 mm × 250 mm, 5 µm) with mobile phases of methanol and 0.6% acetic acid. The drift tube temperature of ELSD was 70 ◦C, and the pressure of nebulizer nitrogen gas was 40 Psi. The method developed has high sensitivity (with limits of detection from 1.3 to 8.6 µg/mL), acceptable linearity over the tested concentrations (with correlation coefficients from 0.991 to 0.999), good repeatability (with intra- and inter-day CV less than 3.1% for all analytes) and satisfactory accuracy (with recovery between 95.5% and 100.8%). The method has been demonstrated as a powerful tool for the functional ingredients analysis and quality control of rosemary and its extracts in a cost- and time-effective manner. © 2019 by the authors

语种： 英文

作者： 金友兰;刘安;肖文军

期刊： 茶叶通讯,2019年46(3):335-339 ISSN：1009-525X

作者机构： 湖南农业大学 茶学教育部重点实验室,国家植物功能成分利用工程技术研究中心,湖南 长沙 410128;湖南省植物功能成分利用协同创新中心,湖南 长沙 410128;[刘安; 金友兰; 肖文军] 湖南农业大学

关键词： 辐照预处理;绿茶茶渣;纤维素酶;水溶性糖

摘要： 以绿茶水提茶渣为原料,水溶性还原糖的含量为考察指标,采用DNS分析方法,在优选60Co辐照降解和纤维素酶酶促水解茶渣制备水溶性糖工艺技术的基础上,探讨了辐照预处理辅助纤维素酶水解绿茶茶渣制备水溶性糖的可行性.结果表明,随着辐照剂量的增大,产糖量显著提高,辐照剂量为1200 kGy时,还原糖含量的增大率为926.01％.不同辐照剂量预处理后的水提茶渣,经酶解处理,其还原糖含量较对照分别提高了1.2倍、1.2倍、1.5倍、1.5倍和1.3倍.由此说明辐照预处理辅助纤维素酶水解绿茶水提茶渣的工艺有利于水溶性糖的制备.

语种： 中文

作者： Li, Penghui;Liu, Ailing;Liu, Changwei;Qu, Zhihao;Xiao, Wenjun;...

期刊： JOURNAL OF NUTRITIONAL BIOCHEMISTRY,2019年74:108225 ISSN：0955-2863

通讯作者： Zhang, Sheng

作者机构： [Liu, Changwei; Liu, Zhonghua; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan; Qu, Zhihao] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Changwei; Liu, Zhonghua; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan; Qu, Zhihao] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Liu, Ailing] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Zhang, Sheng] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

关键词： ephrin;epicatechin;epicatechin gallate;epigallocatechin;epigallocatechin gallate;initiation factor 2;MyoD protein;MyoG protein;myogenic factor;myosin heavy chain;peroxisome proliferator activated receptor alpha;retinoid X receptor alpha;Rho kinase;sirtuin;unclassified drug;catechin;epicatechin gallate;epigallocatechin gallate;gallocatechin;MyoD protein;MyoD1 myogenic differentiation protein;Myog protein, mouse;myogenin;Article;atomic force microscopy;C2C12 cell line;cell adhesion;cell differentiation;controlled study;elasticity;gene expression;muscle development;myoblast;myotube;proteomics;rigidity;signal transduction;skeletal muscle cell;surface property;tea;upregulation;Young modulus;animal;cell differentiation;cell line;chemistry;cytology;drug effect;gene expression regulation;genetics;metabolism;mouse;physiology;skeletal muscle;skeletal muscle cell;skeletal myoblast;Animals;Catechin;Cell Differentiation;Cell Line;Elastic Modulus;Gene Expression Regulation;Mice;Microscopy, Atomic Force;Muscle Fibers, Skeletal;Muscle, Skeletal;Myoblasts, Skeletal;MyoD Protein;Myogenin

摘要： Muscle degeneration and cachexia caused by aging and chronic diseases seriously reduce the quality of life of patients. Catechins in tea enhance skeletal muscle performance, but the specific function and mechanism require further exploration. Myogenic differentiation is accompanied by extensive changes in cell morphology and gene expression patterns. In this study, we measured the number, length, diameter, and transcriptional levels of MyoD, MyoG, and MyHC of myotubes after C2C12 cell differentiation to assess changes in response to four different catechin monomers: epicatechin, epigallocatechin, epicatechin gallate (ECG), and epigallocatechin gallate. The differentiation-promoting effect of ECG was the strongest. Atomic force microscopy showed that ECG significantly reduced the adhesion force, stiffness, and Young's modulus of myotubes. Finally, the mechanism of ECG-enhanced C2C12 cell differentiation was explored by a LC/MS whole proteomic strategy and Ingenuity Pathway Analysis. The results provide a powerful basis for further study of catechin-enhanced skeletal muscle regeneration and repair. © 2019 Elsevier Inc.

语种： 英文

作者： 向奕;刘硕谦;龚志华;陈栋;肖文军

期刊： 茶叶科学,2018年38(5):439-449 ISSN：1000-369X

作者机构： [向奕; 刘硕谦; 龚志华; 陈栋; 肖文军] 湖南农业大学园艺园林学院, 国家植物功能成分利用工程技术研究中心;[向奕; 刘硕谦; 龚志华; 陈栋; 肖文军] 茶学教育部重点实验室;[向奕; 刘硕谦; 龚志华; 陈栋; 肖文军] 湖南省植物功能成分利用协同创新中心, 湖南, 长沙, 410128

关键词： 茶树;花青素;差异基因;转录组测序;生物信息学分析

摘要： 花青素是一类广泛存在于植物中的水溶性色素,作为一种强自由基清除剂,它具有抗炎,抗氧化,降血压,降血糖等多种保健功能。紫芽茶树作为一种高花青素含量的特异性茶树资源,其研究与利用越来越受人们的关注。为了解茶树紫芽中花青素形成的分子机理,本研究通过对湖南农业大学自选紫芽品种9803与绿芽品种9806进行转录组测序及生物信息学分析,筛选得到42条与花青素代谢相关的unigene,其中比对到参考基因组中的有34条,未在参考基因组中登录的有8条。KEGG富集分析表明这42个基因被富集到5个代谢通路中,包括类黄酮合成途径,木质素合成途径,黄酮和黄酮醇合成途径,油菜素甾醇合成途径,以及参与转录因子的编码。通过荧光定量PCR验证,差异基因荧光定量PCR变化趋势与转录组测序结果一致,转录组测序数据可靠。本次试验在转录组水平上筛选出了茶叶紫芽花青素代谢相关的差异基因,为进一步揭示茶叶紫芽产生的分子机理奠定了基础。

语种： 中文

作者： Kang, Jinhe;Yang, Tan;Wu, Zhaoliang;Yang, Jing;Xiong, Min;...

期刊： KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI,2018年24(4):511-517 ISSN：1300-6045

通讯作者： Tan, Zhiliang;Sun, Zhihong

作者机构： [Yang, Tan; Tan, Zhiliang; Kang, Jinhe] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, South Cent Expt Stn Anim Nutr & Feed Sci, Minist Agr,Inst Subtrop Agr,Hunan Res Ctr Livesto, Changsha 410125, Hunan, Peoples R China.;[Yang, Tan; Wu, Zhaoliang; Xiong, Min; Yang, Jing; Sun, Zhihong] Southwest Univ, Key Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.;[Xiao, Wenjun] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Tan, Zhiliang] C;[Sun, Zhihong] S;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, South Cent Expt Stn Anim Nutr & Feed Sci, Minist Agr,Inst Subtrop Agr,Hunan Res Ctr Livesto, Changsha 410125, Hunan, Peoples R China.;Southwest Univ, Key Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.

关键词： caspase 3;caspase 8;cholecystokinin octapeptide;copper zinc superoxide dismutase;glutathione;glutathione peroxidase 1;glutathione transferase;grape seed extract;manganese superoxide dismutase;messenger RNA;peroxiredoxin 5;protein Bax;protein bcl 2;protein p53;thioredoxin;thioredoxin reductase;tumor necrosis factor alpha receptor;analysis of variance;animal cell;animal experiment;animal model;antioxidant assay;antiproliferative activity;apoptosis;Article;B cell lymphoma;cancer cell;cell proliferation;cell survival;comparative study;controlled study;drug efficacy;gene expression;intestine epithelium cell;nonhuman;observational study;oxidation;oxidative stress;porcine model;protein expression;real time polymerase chain reaction;receptor down regulation

摘要： The aim of this study was to investigate the oxidative and proliferative effects of grape seed extract (GSE). Piglet intestinal epithelial cells (IPEC1) were selected as an unstressed cell model, or they were exposed to 400 μM H2O2 to establish a H2O2-stimulated cell model. The glutathione (GSH) and total antioxidant capacity in response to GSE addition were tested in the unstressed and H2O2-stimulated cell models. The relative mRNA levels of antioxidant or antioxidant enzymes and apoptosis-related genes were measured by Real-Time RT-PCR. In the unstressed status, the cell survival ratio and GSH increased with the addition of GSE at 1 and 10 μg/mL but diminished at 60 μg GSE/mL. The addition of 1 μg/mL GSE upregulated the mRNA expression levels of B-Cell Lymphoma protein-2 (Bcl-2), cysteine aspartases-3 (Caspase-3), cysteine aspartases-8 (Caspase-8) and glutathione peroxidase-1(GPx-1), while it downregulated that of Bcl2-associated X protein (Bax), copper-zinc superoxide dismutase, glutathione S-transferase (GST), thioredoxin, thioltransferase and thioredoxin reductase. As GSE reached 60 μg/mL, the tumor protein p53 (p53) and caspase-8 gene expressions were upregulated. In stressed status, 1 and 10 μg GSE/mL promoted the increase of GSH. H2O2-induced increases in Bax, p53, and Caspase-3 mRNA expressions were attenuated by the subsequent addition of 1 μg GSE/mL and promoted the gene expression of tumor necrosis factor-α, GPx-1 and thioltransferase (Ttas). Treatment with 60 μg GSE/ml resulted in a significant reduction in Bax, p53, manganese superoxide dismutase and GST mRNA expressions. These results indicate that GSE exhibits antioxidative and proliferative functions on unstressed IPEC1 cells at low and medium levels and oxidative and antiproliferative functions at high levels. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.

语种： 英文

作者： Li, Chengjian;Yan, Qiongxian*;Tang, Shaoxun;Xiao, Wenjun*;Tan, Zhiliang

期刊： BioMed Research International,2018年2018(10):1-8 ISSN：2314-6133

通讯作者： Yan, Qiongxian;Xiao, Wenjun

作者机构： [Tang, Shaoxun; Yan, Qiongxian; Tan, Zhiliang; Li, Chengjian] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Natl Engn Lab Pollut Control & Waste Utilizat Liv, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha 410125, Hunan, Peoples R China.;[Li, Chengjian] Xiangnan Univ, Coll Pharmaceut Sci, Chenzhou 423000, Peoples R China.;[Li, Chengjian] Yongzhou Vocat Tech Coll, Dept Pharm, Yongzhou 425100, Hunan, Peoples R China.;[Yan, Qiongxian; Xiao, Wenjun; Xiao, WJ] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Tang, Shaoxun; Tan, Zhiliang] CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Yan, Qiongxian] C;[Yan, QX; Xiao, WJ] H;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Natl Engn Lab Pollut Control & Waste Utilizat Liv, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha 410128, Hunan, Peoples R China.

摘要： <jats:p>L-Theanine is a nonprotein amino acid in tea, and its immunomodulatory function has been confirmed. This study aimed to investigate the effect of L-theanine addition on cytokines secretion in rat splenic lymphocytes and explore its potential immunomodulatory effects on the mevalonate biosynthetic pathway. Our results showed that L-theanine treatment did not influence the proliferation and division indexes of the splenic lymphocytes subsets. Interestingly, L-theanine treatment had regulated the contents of IFN-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>γ</mml:mi></mml:mrow></mml:math>, IL-2, IL-4, IL-10, IL-12, and TNF-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mrow><mml:mi>α</mml:mi></mml:mrow></mml:math>  <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mo stretchy="false">(</mml:mo><mml:mi>P</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.001</mml:mn><mml:mo stretchy="false">)</mml:mo></mml:math>except IL-6 and upregulated the mRNA and protein expression of Ras-related protein Rap-1A (Rap1A), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), and farnesyl diphosphate synthase (FDPs)<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mo stretchy="false">(</mml:mo><mml:mi>P</mml:mi><mml:mo>&lt;</mml:mo><mml:mn fontstyle="italic">0.001</mml:mn><mml:mo stretchy="false">)</mml:mo></mml:math>. Additionally, there was a positive correlation between Rap1A and HMGCR proteins expression and IFN-<jats:italic>γ</jats:italic>, IL-4, and IL-6 levels. In conclusion, L-theanine regulated the secretion of cytokines probably by activating expression of Rap1A and HMGCR proteins involved in the mevalonate biosynthetic pathway in rat splenic lymphocytes. Therefore, L-theanine might be a promising potential drug candidate as immunopotentiator.</jats:p>

语种： 英文

作者： 陆英;刘仲华;肖文军;傅冬和;谢红旗;...

期刊： 分析测试学报,2018年37(9):1027-1033 ISSN：1004-4957

作者机构： 湖南农业大学园艺园林学院,湖南长沙410128;湖南农业大学国家植物功能成分利用工程技术研究中心,湖南长沙410128;国家中药材生产(湖南)技术中心,湖南长沙410128;[陆英; 刘仲华; 肖文军; 傅冬和] 湖南农业大学园艺园林学院, 湖南, 长沙, 410128 湖南农业大学, 国家植物功能成分利用工程技术研究中心, 湖南, 长沙, 410128;[谢红旗; 曾建国] 湖南农业大学园艺园林学院, 湖南, 长沙, 410128 国家中药材生产(湖南)技术中心, 湖南, 长沙, 410128

关键词： 分离;中药百合;酚酸甘油酯苷;高速逆流色谱(HSCCC);生物活性

摘要： 建立了高速逆流色谱分离制备药食同源植物百合磷茎中3个酚酸甘油酯苷的方法,同时测定了化合物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除作用、脂质的过氧化抑制作用及对α-淀粉酶活性的促进作用。以乙酸乙酯-正丁醇-水(0.5%乙酸,3 ∶ 1.5 ∶ 5,体积比)为溶剂系统,上相为固定相,下相为流动相,从250 mg百合磷茎提取物中分离得到4.2 mg纯度为96.2%的化合物1,2.3 mg纯度为95.1%的化合物2,5.8 mg纯度为98.8%的化合物3。经质谱及核磁共振鉴定化合物1 ~ 3分别为王百合苷A(Regaloside A) 、乙酰化王百合苷C(Acetylregaloside C) 、王百合苷B(Regaloside B);活性研究表明,Acetylregaloside C对DPPH自由基清除作用及脂质过氧化抑制作用均最强,Regaloside A与Regaloside B对DPPH自由基清除作用很弱,对脂质过氧化有一定的抑制作用且相近,化合物抗氧化作用强弱为:化合物2 > 1≈3。化合物在不同浓度下对α-淀粉酶活性的促进作用具有一定差异,但化合物间无显著差异。

语种： 中文

作者： Deng, Yanli;Han, Xuefeng;Tang, Shaoxun;Li, Chengjian;Xiao, Wenjun*;...

期刊： MEDICAL SCIENCE MONITOR,2018年24:3348-3356 ISSN：1643-3750

通讯作者： Xiao, Wenjun;Tan, Zhiliang

作者机构： [Xiao, Wenjun; Deng, Yanli] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha, Hunan, Peoples R China.;[Deng, Yanli] Guizhou Univ, Tea Coll, Guiyang, Guizhou, Peoples R China.;[Tang, Shaoxun; Tan, Zhiliang; Han, Xuefeng] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Minist Agr, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.;[Tang, Shaoxun; Tan, Zhiliang; Han, Xuefeng] Chinese Acad Sci, Minist Agr, Inst Subtrop Agr, South Cent Expt Stn Anim Nutr & Feed Sci, Changsha, Hunan, Peoples R China.;[Li, Chengjian] Yongzhou Vocat Tech Coll, Dept Pharm, Yongzhou, Hunan, Peoples R China.

通讯机构： [Xiao, Wenjun] H;[Tan, Zhiliang] C;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha, Hunan, Peoples R China.;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Minist Agr, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.;Chinese Acad Sci, Minist Agr, Inst Subtrop Agr, South Cent Expt Stn Anim Nutr & Feed Sci, Changsha, Hunan, Peoples R China.

关键词： dextro amino acid oxidase;gamma interferon;glucose;honokiol;inducible nitric oxide synthase;interleukin 10;lactic acid;loperamide;magnolol;messenger RNA;nitric oxide;autacoid;biphenyl derivative;cytokine;honokiol;lignan;magnolol;nitric oxide synthase;analytical parameters;animal experiment;animal model;animal tissue;apoptosis;Article;blood sampling;concentration (parameters);controlled study;crypt depth;diarrhea;Enterotoxigenic Escherichia coli induced diarrhea;Enterotoxigenic Escherichia coli induced diarrhea;enzyme activity;gastrointestinal absorption;goblet cell;high performance liquid chromatography;histopathology;immunohistochemistry;intestinal secretion;intestine mucosa;male;mouse;nonhuman;protein expression;ratio of villus height to crypt depth;real time polymerase chain reaction;RNA isolation;TUNEL assay;villus height;animal;apoptosis;drug effect;enterotoxigenic Escherichia coli;genetics;homeostasis;intestine absorption;metabolism;microbiology;oral drug administration;pathology;pathophysiology;Administration, Oral;Animals;Apoptosis;Biphenyl Compounds;Cytokines;Enterotoxigenic Escherichia coli;Homeostasis;Inflammation Mediators;Intestinal Absorption;Intestinal Mucosa;Lignans;Mice;Nitric Oxide Synthase

摘要： Background: The cortex of Magnolia officinalis has long been used as an element of traditional Chinese medicine for the treatment of anxiety, chronic bronchitis, and gastrointestinal dysfunction. This study aimed to elucidate the underlying mechanism of its functional ingredients (magnolol and honokiol) in modifying the secretion and absorption homeostasis and protecting mucosal integrity in an Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea mouse model. Material/Methods: This study established a diarrhea mouse model infected by ETEC at a dosage of 0.02 ml/g live body weight (BW) in vivo. Magnolol or honokiol was followed by an intraperitoneal administration at dosages of 100, 300, and 500 mg/kg BW according to a 3×3 factorial arrangement. The useful biomarkers for evaluating the integrity of intestinal tract and histologic injury were analyzed and morphological development (including villus height, crypt depth, and ratio of villus height to crypt depth) and the expressions of inflammatory cytokines were determined by real-time PCR. Results: The results showed that magnolol and honokiol (500 mg/kg BW) reduced the concentrations of NO, DAO, and DLA, and iNOS activity, and the mRNA expressions of the interferon gamma (IFN-γ) and interleukin 10 (IL-10), and inhibited intestinal epithelial cell apoptosis. Magnolol and honokiol (300 mg/kg BW) elongated the villus height and crypt depth and decreased the number of goblet cells and the ratio of villus height to crypt depth. Conclusions: The current results indicate that magnolol and honokiol enhance the intestinal anti-inflammatory capacities, elongate the villus height and crypt depth, and reduce goblet cell numbers to inhibit the intestinal epithelium apoptosis and effectively protect the intestinal mucosa. These results show that magnolol and honokiol protect the intestinal mucosal integrity and regulate gastrointestinal dysfunction. © Med Sci Monit, 2018.

语种： 英文

作者： 刘林峰;向奕;刘安;肖文军;龚志华

期刊： 茶叶通讯,2018年45(1):3-8 ISSN：1009-525X

作者机构： 湖南农业大学 茶学教育部重点实验室,湖南省植物功能成分利用协同创新中心,湖南 长沙 410128;[向奕; 刘安; 龚志华; 肖文军; 刘林峰] 湖南农业大学

关键词： 茶叶;紫芽;花青素

摘要： 随着天然色素保健功能的深入研究,茶叶花青素也逐渐受到广泛关注。本文从花青素的化学结构与理化性质、茶树紫芽花色苷代谢机制及富集特性、茶叶花色苷定性定量分析方法、茶叶花青素提取分离与纯化方法、生物活性及产品开发等方面进行综述,为茶叶花青素及其苷的综合开发提供参考。

语种： 中文

作者： Gong, Zhihua;Liu, Qiuling;Lin, Ling;Deng, Yanli;Cai, Shuxian;...

期刊： European Journal of Pharmacology,2018年818:184-190 ISSN：0014-2999

通讯作者： Xiao, Wenjun;Xiong, Shuo;Chen, Dong

作者机构： [Xiao, WJ; Xiong, S; Chen, D; Gong, Zhihua; Xiong, Shuo; Liu, Zunying; Chen, Dong; Xiao, Wenjun; Zhang, Sheng; Liu, Qiuling; Cai, Shuxian; Lin, Ling; Deng, Yanli] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Gong, Zhihua; Xiong, Shuo; Liu, Zunying; Chen, Dong; Xiao, Wenjun; Zhang, Sheng; Liu, Qiuling; Cai, Shuxian; Lin, Ling; Deng, Yanli] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Xiong, Shuo] Chinese Acad Sci, Inst Bont, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China.;[Deng, Yanli] Guizhou Univ, Tea Coll, Guiyang 550025, Guizhou, Peoples R China.;[Chen, Dong] Guangdong Acad Agr Sci, Tea Res Inst, Guangzhou 510640, Guangdong, Peoples R China.

通讯机构： [Xiao, WJ; Xiong, S; Chen, D] H;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.

关键词： Apoptosis response;ETEC;Hepatic injury;l-Theanine

摘要： L-Theanine (LTA; γ-glutamylethylamide), a peculiar non-protein-derived amino acid isolated from tea, is widely used as a functional ingredient and dietary supplement. L-Theanine has been confirmed to have hepatoprotective effects, but the underlying mechanism remains unknown. This study investigated the protective effect of L-Theanine-in vivo, using an enterotoxigenic Escherichia coli (ETEC)-infected mouse model. L-Theanine significantly decreased the elevated serum activities of both aspartate aminotransferase (AST) and alanine aminotransferase (ALT), two biomarkers of hepatic impairment. This was consistent with histopathological images from the microscopic observation of liver tissue. In addition, L-theanine significantly increased the mRNA and protein expression of Bcl-2 and decreased the expression of Bax, anti- and pro-apoptotic molecules, respectively, compared with levels in the ETEC control group. The expression of cleaved caspase-3 protein in the group pre-treated with L-theanine was significantly lower than that in the ETEC group. Additionally, decreases in extracellular signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase(JNK1/2) MAPK phosphorylation were observed in the L-theanine pre-treated group. Our study demonstrates that L-theanine possesses anti-apoptotic activity, which can be attributed to suppression of the intrinsic mitochondria-mediated apoptosis and MAPK phosphorylation signaling pathways. © 2017 Elsevier B.V.

语种： 英文

作者： Li, Chengjian;Yan, Qiongxian*;Tang, Shaoxun;Xiao, Wenjun;Tan, Zhiliang

期刊： MEDICAL SCIENCE MONITOR,2018年24:2109-2118 ISSN：1643-3750

通讯作者： Yan, Qiongxian

作者机构： [Tang, Shaoxun; Yan, Qiongxian; Tan, Zhiliang; Li, Chengjian] Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Stn Anim Nutr & Feed Sci,Minist Agr,Inst Subtrop, Changsha, Hunan, Peoples R China.;[Li, Chengjian] Xiangnan Univ, Coll Pharmaceut Sci, Chenzhou, Hunan, Peoples R China.;[Li, Chengjian] Yongzhou Vocat Tech Coll, Dept Pharm, Yongzhou, Hunan, Peoples R China.;[Yan, Qiongxian; Xiao, Wenjun] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha, Hunan, Peoples R China.;[Tang, Shaoxun; Tan, Zhiliang] CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.

通讯机构： [Yan, Qiongxian] C;[Yan, Qiongxian] H;Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Stn Anim Nutr & Feed Sci,Minist Agr,Inst Subtrop, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Prov Coinnovat Ctr Utilizat Bot Funct Ingredients, Changsha, Hunan, Peoples R China.

关键词： caspase 3;fluorescein isothiocyanate;glutathione;glutathione peroxidase;hydrogen peroxide;lipocortin 5;mitogen activated protein kinase p38;nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase;nitric oxide;penicillin derivative;reactive oxygen metabolite;streptomycin;stress activated protein kinase;superoxide dismutase;theanine;antioxidant;Casp3 protein, rat;glutamic acid derivative;glutathione;glutathione peroxidase;hydrogen peroxide;protective agent;superoxide dismutase;theanine;animal cell;antioxidant activity;apoptosis;Article;cell culture;cell damage;cell proliferation;cell structure;cell survival;cell viability;controlled study;flow cytometry;fluorescence microscopy;heart disease;nonhuman;oxidative stress;protein expression;protein phosphorylation;rat;reperfusion injury;signal transduction;upregulation;Western blotting;animal;apoptosis;cardiac muscle cell;cell line;drug effect;MAPK signaling;metabolism;phosphorylation;Animals;Antioxidants;Apoptosis;Caspase 3;Cell Line;Cell Survival;Glutamates;Glutathione;Glutathione Peroxidase;Hydrogen Peroxide;JNK Mitogen-Activated Protein Kinases;MAP Kinase Signaling System;Myocytes, Cardiac;Oxidative Stress;p38 Mitogen-Activated Protein Kinases;Phosphorylation;Protective Agents;Rats;Reactive Oxygen Species;Signal Transduction;Superoxide Dismutase

摘要： Background: L-theanine is a non-protein amino acid in green tea, and its hepatoprotection and neuroprotection have been verified. However, whether L-theanine can prevent cardiomyocytes from apoptosis is unclear yet. This study evaluated the protective effects of L-theanine on H2O2-induced heart injury in vitro. Material/Methods: The certified H9C2 cells were pretreated with L-theanine (0 mM, 4 mM, 8 mM, and 16 mM) for 24 h, followed by 160 μM H2O2 solution for 4 h. The cell viability and antioxidant indices were assayed. Quantitative evaluation of apoptosis was performed by flow cytometric analysis. Nuclear morphology of the cells was monitored by 4’,6-diamidino-2-phenylindole staining. Expression of Caspase-3, poly ADP-ribose polymerase (PARP), c-Jun N-terminal kinase (JNK), and mitogen-activated protein kinase p38 was assayed by Western blot. Results: Compared to the H2O2 treatment, all doses of L-theanine treatments increased the cell viability, glutathione level, and the activities of glutathione peroxidase and superoxide dismutase (P&lt;0.001). The contents of reactive oxygen species, nitric oxide, and oxidized glutathione were decreased by L-theanine treatments (P&lt;0.001). Meanwhile, L-theanine treatments decreased the apoptosis ratio of H2O2-induced H9C2 cells (P&lt;0.001). Pro- Caspase-3 expression was upregulated and cleavaged-PARP expression was inhibited by L-theanine (P&lt;0.001). However, the phosphorylation of JNK and p38 was not affected by L-theanine treatments (P&gt;0.05). Conclusions: These data indicate that L-theanine pretreatment prevents H2O2-induced apoptosis in H9C2 cells, probably via antioxidant capacity improvement. Therefore, it might be a promising potential drug candidate for prophylaxis of ischemia/reperfusion-induced heart diseases. © Med Sci Monit.

语种： 英文

作者： 郭晨;刘林峰;向奕;罗莉;林玲;...

期刊： 茶叶科学,2017年37(1):17-24 ISSN：1000-369X

作者机构： 湖南农业大学园艺园林学院, 茶学教育部重点实验室;[郭晨; 刘林峰; 向奕; 罗莉; 林玲] 国家植物功能成分利用工程技术研究中心, 湖南, 长沙, 410128;[肖文军] 国家植物功能成分利用工程技术研究中心;[肖文军] 湖南省植物功能成分利用协同创新中心, 湖南, 长沙, 410128;[郭晨; 刘林峰; 向奕; 罗莉; 林玲; 肖文军] 湖南农业大学园艺园林学院, 茶学教育部重点实验室

会议名称： 2016湖南茶业科技创新论坛

会议时间： 2016-12-01

会议地点： 长沙

会议论文集名称： 2016湖南茶业科技创新论坛论文集

关键词： L-茶氨酸;热处理;组织损伤;氧化逆境

摘要： 以SPF级Slca/KM雄性小鼠作为实验对象,在恒温恒湿模拟气候箱进行热处理,灌喂干预不同剂量L-茶氨酸,考察L-茶氨酸对小鼠采食量、体重变化、空肠切片病理情况、器官指数、血清谷丙转氨酶(ALT)与谷草转氨酶(AST)活性、血清肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)和干扰素-γ(IFN-γ)含量、肝组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及肝组织病理学变化的影响,以探讨L-茶氨酸改善热处理引起的小鼠组织损伤和氧化逆境作用。结果表明,L-茶氨酸能在一定程度上促进热处理小鼠采食量和体重的增加,降低肝脏和脾脏器官指数,降低血清ALT、AST酶活性,抑制血清炎症介质TNF-α、IL-6、 IFN-γ的含量,降低肝组织MDA含量,提高SOD、GSH-Px、CAT酶活性,减轻热处理对空肠及肝脏组织的损伤,且以L-茶氨酸中剂量处理效果较好。说明L-茶氨酸具有改善热处理引起的小鼠组织损伤和氧化逆境的作用,主要表现为提高小鼠营养物质吸收能力、减少炎性细胞因子表达,以及降低氧化损伤。

语种： 中文