作者： Wang, Yan;Salem, Abdelfattah Z. M.;Tan, Zhiliang;Kang, Jinhe*;Wang, Zheng*

期刊： ITALIAN JOURNAL OF ANIMAL SCIENCE,2021年20(1):195-204 ISSN：1594-4077

通讯作者： Kang, Jinhe;Wang, Zheng

作者机构： [Wang, Zheng; Kang, Jinhe; Wang, Yan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Tan, Zhiliang; Wang, Yan; Kang, Jinhe] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, South Cent Expt Stn Anim Nutr & Feed Sci,Minist A, Changsha, Hunan, Peoples R China.;[Wang, Yan] Hunan Univ Humanities Sci & Technol, Coll Agr & Biotechnol, Loudi, Hunan, Peoples R China.;[Tan, Zhiliang; Wang, Yan; Kang, Jinhe] CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Salem, Abdelfattah Z. M.] Univ Autonoma Estado Mexico, Fac Med Vet & Zootecnia, Mexico City, DF, Mexico.

通讯机构： [Kang, JH; Wang, Z] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.

关键词： Antioxidant response;high-concentrate diet;nuclear factor E2-related factor 2;glucocorticoid receptor

摘要： This study investigated changes in oxidative stress and the relevant mechanisms in the liver of goats fed a high-concentrate (HC) diet for 5 weeks. Twelve goats were randomly assigned to a low-concentrate (concentrate-to-forage = 55:45, LC, n = 6) or HC diet (concentrate-to-forage = 90:10, n = 6), with dry matter as the base. Enzyme activity assays, real-time polymerase chain reaction and western blotting were used to evaluate antioxidant parameters and gene expression in the liver. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), total nitric oxide synthase activity and total antioxidant capacity (T-AOC) in the liver declined (p < .01) in HC-fed goats compared to those in LC-fed goats. The mRNA levels of GPX1, CAT and SOD1 were down-regulated (3.69, 47.37 and 27.61%, respectively) in HC-fed goats compared to those in LC-fed goats. Furthermore, glutathione S-transferase M1 (GSTM1) was upregulated (466.35%, p < .01) in the liver of HC-fed goats. The mRNA and protein levels of the nuclear factor E2-related factor 2 (NRF2) and total glucocorticoid receptor (GR) declined (p < .05) in HC-fed goats (by 28.57, 33.1, 30.85 and 34%, respectively). However, the nuclear translocation of GR increased (p < .05; by 44.75%) in HC-fed goats. Negative correlations were detected for hepatic nuclear GR protein expression with hepatic CAT activity and GPx activity. In conclusion, feeding an HC diet to goats for 5 weeks suppressed NRF2-dependent antioxidant responses and enhanced GR nuclear translocation in the liver. Feeding goats high-concentrate diets suppresses NRF2-dependent antioxidant responses in liver, downregulating NRF2 and altering SOD1, GPx and CAT activity. This suppression of NRF2-dependent antioxidant responses is associated with GR activation in the liver of goats.

语种： 英文

作者： Wang, Juan;Li, Qinjin;Peng, Yuande;Yang, Huilin;Wang, Zhi*;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2019年112(1):335-340 ISSN：0022-0493

通讯作者： Wang, Zhi

作者机构： [Peng, Yuande; Wang, Zhi; Wang, Juan] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Yang, Huilin; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Li, Qinjin; Luo, Lin] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.;[Li, Qinjin] Hunan Womens Univ, Tourism Dept, Changsha 410000, Hunan, Peoples R China.;[Wang, Zhi] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] C;[Wang, Zhi] H;Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

关键词： PQCI sensor;Pardosa pseudoamulata;methamidophos;proteinase

摘要： A piezoelectric quartz crystal impedance (PQCI) sensor was used to investigate influences of the insecticide methamidophos on proteinase activity in midguts of the wolf spider, Pardosa pseudoamulata (Araneae: Lycosidae). Results from PQCI indicated that low-concentration dose methamidophos (0.008%) can activate the proteinase but high-concentration dose methamidophos (0.016-0.032%) can inhibit the enzyme activity. The changes in subcellular structure of spider midgut cells were also observed. Electron micrographs of spider midgut epithelial cells showed that the low-dose methamidophos did not visibly impact the structure of these cells. Conversely, high-concentration dose methamidophos led to severe changes in the cell structure, including the karyotheca dissolved, the nucleolus, and the endoplasmic reticulum disappeared. These may contribute to changes in proteinase activity of spider. This work documents a feasible method for rapid and reliable detection of proteinase activity.

语种： 英文

作者： Zhang, Jing;Wang, Qiming;Zhu, Zhonglin;Qian, Hongliang;Jiang, Feng;...

期刊： Chemical Engineering & Technology,2019年42(2):388-396 ISSN：0930-7516

通讯作者： Huang, Dechun;Liu, Wei

作者机构： [Zhang, Jing; Jiang, Feng; Wang, Zhixiang; Qian, Hongliang; Zhu, Zhonglin; Huang, Dechun] China Pharmaceut Univ, Sch Engn, 639 Longmian Ave, Nanjing 210009, Jiangsu, Peoples R China.;[Wang, Qiming] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Liu, Wei] Nanjing Normal Univ, Sch Energy & Mech Engn, 1 Wenyuan Rd, Nanjing 210009, Jiangsu, Peoples R China.

通讯机构： [Huang, Dechun] C;[Liu, Wei] N;China Pharmaceut Univ, Sch Engn, 639 Longmian Ave, Nanjing 210009, Jiangsu, Peoples R China.;Nanjing Normal Univ, Sch Energy & Mech Engn, 1 Wenyuan Rd, Nanjing 210009, Jiangsu, Peoples R China.

关键词： Cosolvent;Gefitinib;Microparticle;Solubility;Solution-enhanced dispersion by supercritical fluids

摘要： The antineoplastic gefitinib has a low aqueous solubility, leading to poor absorption rates. To overcome this problem, microparticles (MP) were prepared using solution-enhanced dispersion by supercritical fluids (SEDS) with supercritical CO2 and the cosolvents dichloromethane and ethanol. The results showed that the use of SEDS resulted in the formation of smaller particles and rendered the usually heterogeneous crystals of the crude drug pure and uniform. Furthermore, the reduction in the MP size increased the dissolution rate of gefitinib, and in vitro cytotoxicity assays indicated that the MP inhibited the proliferation of A549 cells to a larger extent than did the crude drug. Given the beneficial properties of the MP, SEDS could potentially be used to micronize drugs for therapeutic applications. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

语种： 英文

作者： 刘沙彦;彭曙光;罗井清;周志成;王征

期刊： 江苏农业科学,2019年(04):171-175 ISSN：1002-1302

作者机构： 1. 湖南农业大学生物科学技术学院;2. 中国烟草中南农业试验站

关键词： 绿原酸;包埋;多孔淀粉;卵磷脂;缓释性

摘要： 为提高绿原酸的生物利用度,采用多孔淀粉对绿原酸进行包埋,再用卵磷脂对绿原酸-多孔淀粉包埋物进行包裹,形成复合包埋物。通过单因素和正交试验对其吸附绿原酸的条件进行优化,得到最佳包埋条件:芯壁比为1∶10,pH值为3,温度为30℃,时间为2.0 h,包埋效率达到82.5%,包埋量为82.4 mg/g。通过搅拌将卵磷脂和绿原酸-多孔淀粉包埋物混合形成卵磷脂复合包埋物,其包埋效率仍为82.5%。分别利用傅里叶红外、扫描电镜和紫外扫描对包埋物进行了分析和确证。将2种包埋物分别于人工模拟胃液和肠液环境中进行绿原酸释放研究,结果显示卵磷脂复合包埋物在人工模拟胃液中的最高释放率为16.3%,在人工模拟肠液中的最高释放率为59.2%,而绿原酸-多孔淀粉包埋物在上述2种环境下的释放率分别为64.3%和35.2%。表明卵磷脂复合物能更好地经受胃液环境,到达肠液中释放绿原酸,也表明该包埋工艺可以提高绿原酸的生物利用度。

语种： 中文

作者： 许诗豪;彭曙光;罗井清;杨佳蒴;王征

期刊： 中国农学通报,2019年35(1):57-62 ISSN：1000-6850

作者机构： 湖南农业大学生物科学技术学院,长沙,410128;湖南省烟草科学研究所,长沙,410007

关键词： 绿原酸;芸香苷;响应面设计;高效液相色谱

摘要： 旨在获得合适的绿原酸与芸香苷提取工艺,提高低次烟叶的经济附加值.笔者采用单因素试验和响应面设计,高效液相色谱法检测提取量,选择乙醇浓度、料液比、提取时间、提取温度4个因素优化提取工艺.结果表明,绿原酸与芸香苷最佳提取工艺条件为:乙醇浓度67％,料液比1∶16 (g:mL),提取温度61℃,提取时间150 min.此条件下,绿原酸与芸香苷理论提取量之和为43.59 mg/g,相对误差0.36％.使用Design-Expert软件对4个因素进行分析,其影响大小顺序依次为:乙醇浓度＞提取时间＞料液比＞提取温度.本研究获得了从低次烟叶中提取绿原酸与芸香苷的工艺条件,为更好利用低次烟叶提供了新思路.

语种： 中文

作者： 陈思维;王征

期刊： 中国辐射卫生,2019年28(04):447-451 ISSN：1004-714X

作者机构： 湖南农业大学生物科学技术学院,湖南长沙,410128;[陈思维; 王征] 湖南农业大学

关键词： 绿原酸;废弃烤烟叶;提取过程;动力学仿真模型

摘要： 目的 通过仿真模型复现烟叶绿原酸提取过程的含量变化规律.方法 基于天然药物提取过程中的动力学数学模型;建立低次烟叶绿原酸提取动力学方程在matlab平台的simulink仿真框图模型,并通过实验的方法确定模型参数.结果 运行仿真框图模型获得预测提取数据的曲线.通过在50℃、60℃、70℃三个温度梯度下,测定对应时间下的提取浓度,验证了仿真模型的准确性.结论 仿真模型可以在不同温度及不同时刻下,给出提取过程中绿原酸含量的大致变化规律,为绿原酸的提取工艺研究提供参考依据.

语种： 中文

作者： 王艳;董鹏;金晨钟;康劲翮;胡一鸿;...

期刊： 基因组学与应用生物学,2019年38(5):2191-2199 ISSN：1674-568X

作者机构： [王艳] 湖南农业大学生物科学技术学院,长沙,410128;[王艳] 湖南人文科技学院,农田杂草防控技术与应用协同创新中心,娄底,417000;中国科学院亚热带农业生态过程重点实验室,长沙,410125;[董鹏; 金晨钟; 胡一鸿] 湖南人文科技学院;[王征] 湖南农业大学

关键词： 黄精多糖;气相色谱质谱联用仪;单糖组成;抗氧化活性

摘要： 分离纯化湖南新化产黄精多糖,并对其组分、结构特征和抗氧化活性进行研究。本研究采用碱提法、 Sevag法脱蛋白得水溶性粗多糖(PSP),并经DEAE-52和Sepharose CL-6B色谱柱分离纯化得黄精多糖分级组分。通过紫外、红外光谱和GC-MS进行初步结构分析,并检测其体内外抗氧化能力。结果PSP经纯化得1个组分PSP-1a,不含蛋白质和核酸,具有典型多糖吸收峰,主要由半乳糖、阿拉伯糖、鼠李糖、木糖、葡萄糖组成,其摩尔百分含量分别为0.18%、1.50%、97.25%、0.77%和0.3%。体外抗氧化活性研究表明:PSP和PSP-1a均具有一定抗氧化性能力,且随浓度(1~10 mg/L)增大而增强,PSP-1a的抗氧化能力优于PSP,但弱于VC。体内实验显示,PSP-1a改善了高脂膳食肥胖小鼠结肠匀浆组织的氧化应激状况。

语种： 中文

作者： Wang, Juan;Lv, Zhiyue;Lei, Ziyan;Chen, Zhaoyang;Lv, Bo;...

期刊： Ecotoxicology and Environmental Safety,2019年172:19-25 ISSN：0147-6513

通讯作者： Wang, Zhi

作者机构： [Wang, Zhi; Wang, Juan] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Yang, Huilin; Chen, Zhaoyang; Lei, Ziyan; Lv, Bo; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

关键词： Cd transporters;Cuticle;Cytochrome P450 enzymes;Ecdysteroids;Molting

摘要： Cytochrome P450 enzymes (CYPs), encoded by Halloween genes, mediate the biosynthesis of molting hormone, ecdysteroids, in arthropods. In this report, the effect of heavy metal cadmium (Cd) stress on the expression of cytochrome P450 genes in the wolf spider Pardosa pseudoannulata was analyzed. The results showed the expression levels of genes encoding for Cd transporters including ABC transporters, zinc transporters, calcium channel proteins and calcium binding proteins were inhibited or induced by Cd stress. In addition, the increase in metallothionein (MT) content and glutathione peroxidase (GPX) activity and decrease in total acetylcholine esterase (AChE) activity were also detected. Apparently, these detoxification methods did not completely protect the spider from the cytotoxicity of Cd stress. Increased mortality of P. pseudoannulata was observed when they were under Cd tress. In total 569 CYP genes belonging to 62 CYP subfamilies were obtained from P. pseudoannulata RNA-seq databases. BlaxtX analysis showed that 150, 161, 11, and 40 CYP genes were similar to the genes dib, phm, sad and shd, respectively, which are thought to catalyze the biosynthesis of ecdysteroids. Gene expression analysis suggested that 25 dib encoding genes, 27 phm encoding genes, 2 sad encoding genes, and 6 shd encoding genes were differentially expressed in TS2 vs. S2 comparison (Cd-treated 2nd instar spider vs. 2nd instar spider), respectively. There were 70 dib, 70 phm and 19 shd encoding genes either upregulated or downregulated, while 3 sad encoding genes were upregulated in TS5 vs. S5 (Cd-treated 5nd instar spider vs. 5nd instar spider). Genes related to heme binding and essential for activating the CYPs were also differentially expressed. Expression levels of cuticle related genes were significant differentially expressed, implying the changes in activities of chitin synthases and chitinase. Therefore we assume that unsuccessful molting process may occur on P. pseudoannulata due to influenced ecdysteroids levels, thus increasing mortality of spider.

语种： 英文

作者： Yang, Huilin;Peng, Yuande;Shi, Yixue;Tian, Jianxiang;Wang, Juan;...

期刊： Environmental Science and Pollution Research,2018年25(24):23875-23882 ISSN：0944-1344

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin; Wang, Zhi; Shi, Yixue; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Changsha 410006, Hunan, Peoples R China.

关键词： Cadmium;Transcriptomic;P. pseudoannulata;Venom glands;Innate immunity

摘要： Due to some similarity of innate immunity between insects and mammals, the study of the molecular mechanism of innate immunity in insects has become a focus of research. However, the exact molecular and cellular basis of immune system in insect remains poorly understood. Characterization of the transcriptomic response to Cd of spider is an effective approach to understanding the innate immunity mechanisms. In this study, we carried out transcriptome sequencing and gene expression analyses to develop molecular resources for Pardosa pseudoannulata venom glands with and without Cd treatments. A total of 92,778 assembled unigenes and 237 Cd stress-associated differentially expressed genes between the Cd-treated and control groups were obtained. Expression profile analysis demonstrated that immunity-related genes involved in bacterial invasion of epithelial cells, leukocyte transendothelial migration, platelet activation, apoptosis, phagosome, and Rap1 signaling pathway were upregulated by Cd exposure, except the genes involved in PPAR signaling pathway were downregulated. Our results provide the first comprehensive transcriptome dataset of venom glands in P. pseudoannulata response to Cd, which is valuable for throws light on the immunotoxicity mechanism of Cd, and the innate immunity complexity. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Zou, Wansheng;Wang, Zhi*;Song, Qisheng;Tang, Shaoxian;Peng, Yuande

期刊： Harmful Algae,2018年77(1):18-28 ISSN：1568-9883

通讯作者： Wang, Zhi

作者机构： [Wang, Zhi; Zou, Wansheng; Tang, Shaoxian] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 NongdaRd, Changsha 410128, Hunan, Peoples R China.;[Zou, Wansheng] Hunan Univ Arts & Sci, Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Hunan, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.;[Peng, Yuande] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Wang, Zhi] Hunan Normal Univ, Sch Life Sci, Changsha 410000, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 NongdaRd, Changsha 410128, Hunan, Peoples R China.

关键词： *Benthic bacteria;*Microcystis aeruginosa;*Recruitment-promoting;*Sediment

摘要： The frequent occurrence of Microcystis aeruginosa blooms benefit from the dormant Microcystis cells, which will be recruited from sediment into overlying water to form a dominant population and algal blooms when external environmental conditions are suitable. Previous studies have unveiled factors involved in M. aeruginosa recruitment and bloom initiation, including nutrition, illumination, temperature, and hydrodynamic force. In this study, three dominant benthic bacterial species isolated from Lake Chongtian with frequent blooms-forming were identified through next generation sequencing (NGS) techniques, and laboratory experiments were conducted on the recruitment of dormant M. aeruginosa cells via co-culture with these bacteria at 10 °C, 15 °C, 20 °C and 25 °C. The results showed that the bacterial strains in sediment proliferated quickly before recruitment of dormant M. aeruginosa cells, subsequently significantly promoted the recruitment of dormant M. aeruginosa via allelochemical (metabolite) production, lower N:P values and lower dissolved oxygen concentrations in the sediment-water interface, and enhanced photosynthesis of M. aeruginosa cells. Furthermore, dormant M. aeruginosa was recruited from sediment at 10 °C when bacterial activity was present, but not recruited when bacterial activity was absent. At 15 °C,20 °Cand 25 °C, there were no remarkable differences in the recruitment rate of dormant M. aeruginosa cells among all bacterial groups, although their recruitment rate were significantly higher than that at 10 °C.These findings suggested that, under laboratory conditions, three benthic bacteria not only had a great influence on promoting the recruitment of dormant M. aeruginosa cells under desirable temperatures, but also can spur recruitment of dormant M. aeruginosacells from sediment at lower temperature (10 °C). © 2018 Elsevier B.V.

语种： 英文

作者： Wang, Juan;Peng, Yuande;Wang, Zhi*;Chai, Xiangping*;Lv, Zhiyue;...

期刊： Biologia,2018年73(11):1099-1108 ISSN：0006-3088

通讯作者： Wang, Zhi;Chai, Xiangping

作者机构： [Wang, Zhi; Wang, Juan] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Wang, Zhi] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Chai, Xiangping] Cent S Univ, Xiangya Hosp 2, Emergency Dept, Changsha 410011, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.

通讯机构： [Wang, Zhi] H;[Wang, Zhi; Chai, Xiangping] C;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;Cent S Univ, Xiangya Hosp 2, Emergency Dept, Changsha 410011, Hunan, Peoples R China.

关键词： Heterometrus liangi;Crude venom;HeLa cells;Cell proliferation;Apoptosis;p21 protein

摘要： Scorpion venoms are important sources for identifying and developing promising biomolecules for cancer treatment. To search for promising biomolecules for cancer research, the effects of crude venom from the scorpion Heterometrus liangi (HLSN) on the proliferation, apoptosis and underline molecular mechanisms of HeLa cells were investigated and compared with that of Buthus martensii (BMK). RT-PCR, Western blot and immunohistochemical staining were used to evaluate the expression levels of p21 in the control and treated cells. The present data showed that when HeLa cells were exposed to HLSN venom for 24 and 48 h, the venom inhibited HeLa cell proliferation in a dose-dependent manner as determined by the methyl thiazolyl tetrazolium (MTT) assay, caused marked morphological changes as revealed by hematoxylin and eosin (HE) staining, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and induced apoptosis as shown by flow cytometry (FCM). Western blot and RT-PCR analysis revealed that the expression of p21 protein was upregulated at both transcriptional and translational levels, as supported by immunohistochemical staining. Therefore, HLSN crude venom, similar to BMK venom, inhibits proliferation and induces apoptosis in HeLa cells via the induction of p21 expression while it has little adverse effect on non cancer human umbilical vein endothelial (HUVEC-21) cells. © 2018, Institute of Zoology, Slovak Academy of Sciences.

语种： 英文

作者： Yang, Huilin;Wang, Juan;Lv, Zhiyue;Tian, Jianxiang;Peng, Yuande;...

期刊： Ecotoxicology and Environmental Safety,2018年159:1-9 ISSN：0147-6513

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin; Chen, Zhaoyang; Wang, Zhi; Lv, Bo; Sun, Zhiying; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： Cadmium;Intestinal microorganisms;Metatranscriptome;P. pseudoannulata

摘要： Cadmium (Cd) generates a variety of physiological and ecological toxicity to spiders. However, little is known about the effects of Cd on symbiotic bacteria of spiders. Metatranscriptomics is increasing our knowledge of microorganisms in environment. To better understand the impact of Cd on the symbiotic bacteria of spiders, we generated and compared the metatranscriptomes of the intestinal microorganisms of Pardosa pseudoannulata with and without Cd stress. The community structure of intestinal microorganisms in P. pseudoannulata was composed of 4 kingdoms, namely bacteria, viruses, eukaryotes and archaea, including 46 phyla, 97 classes, 184 orders, 339 families, 470 genera, and 598 species. The abundance of eukaryotes, bacteria and viruses was decreased by 0.14%, 1.22% and 2.52% respectively while the archaea was increased by 99.16% when under Cd stress. We identified 1519 differentially expressed genes (DEGs), including 770 up-regulated and 749 down-regulated genes. The results of KEGG annotation revealed that the expression of genes that are involved in the carbon metabolism, protein and amino acid metabolism and synthesis, glucose metabolism, oxidative phosphorylation, and glutathione metabolism were influenced by Cd. Collectively, these findings showed that Cd significantly impacted the community structure and expression of related functional genes of intestinal microorganisms in P. pseudoannulata. © 2018 Elsevier Inc.

语种： 英文

作者： Song, Wei;Jiang, Meijie;Wang, Zongling*;Wang, Hongping;Zhang, Xuelei;...

期刊： 海洋学报(英文版),2018年37(4):102-108 ISSN：0253-505X

通讯作者： Wang, Zongling

作者机构： [Wang, Zongling; Wang, Hongping; Fu, Mingzhu; Jiang, Meijie; Zhang, Xuelei; Song, Wei] State Ocean Adm, Key Lab Sci & Engn Marine Ecol & Environm, Inst Oceanog 1, Qingdao 266061, Peoples R China.;[Wang, Zongling; Wang, Hongping; Fu, Mingzhu; Jiang, Meijie; Zhang, Xuelei; Song, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266235, Peoples R China.;[Song, Wei] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Zongling] S;[Wang, Zongling] Q;State Ocean Adm, Key Lab Sci & Engn Marine Ecol & Environm, Inst Oceanog 1, Qingdao 266061, Peoples R China.;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266235, Peoples R China.

关键词： green tides;source of propagules;Ulva prolifera;anti-fouling

摘要： Since 2007, large-scale green tides dominated by Ulva prolifera consecutively bloomed in the Yellow Sea and caused great economic losses. The fouling U. prolifera on the Pyropia yezoensis aquaculture rafts in the Subei Shoal was regarded as the major source of the floating biomass. However, it was still unclear about the seed source of fouling green macroalgae attached on the rafts. In this study, the field surveys and the indoor experiments were conducted to reveal the source of propagules of the fouling green macroalgae on the rafts and to study the anti-fouling material for P. yezoensis aquaculture rafts which could possibly be a feasible strategy to control the green tides in the Yellow Sea. The results showed that (1) micro-propagules of several green macroalgal species, including U. prolifera, U. linza, U. compressa, U. flexuosa, and Blidingia sp. coexisted in the waters and sediments in the Subei Shoal and their proportion remarkably changed over time; (2) the bamboo poles with peeling treatment could significantly reduce the amount of U. prolifera micro-propagules attached. This study confirmed that the micro-propagules distributed in the Subei Shoal area were the precursors of the green tides, and provided a feasible method to control the Yellow Sea large-scale green tides at the beginning. © 2018, The Chinese Society of Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Yang, Huilin;Peng, Yuande;Tian, Jianxiang;Wang, Juan;Wei, Baoyang;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2018年111(1):53-64 ISSN：0022-0493

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin; Wang, Zhi; Wei, Baoyang; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： "rice field spider";"study advances";China;"predation function"

摘要： Many laboratory and field studies have been conducted on rice field spiders in China. There are 375 species, 108 genera, and 22 families of rice field spiders distributed within the major rice growing areas and 17 dominant species. The biological and ecological characteristics of 17 rice field spider species have been reported in detail. The biology and ecology of these species show significant differences among regions, farmland habitats, and agricultural practices. Future research should focus on rice field habitat diversity, enhancing the insecticide resistance of dominant spider populations, implementing large-scale breeding of spiders and augmentative release, breeding more leaf dominant species, conducting biosafety assessment of spiders in transgenic crops.

语种： 英文

作者： Peng, Shu-guang;Pang, Yi-lin;Zhu, Qi;Kang, Jing-he;Liu, Ming-xin;...

期刊： BioMed Research International,2018年2018:8594767 ISSN：2314-6133

通讯作者： Wang, Zheng

作者机构： [Liu, Ming-xin; Wang, Zheng; Pang, Yi-lin; Peng, Shu-guang; Zhu, Qi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Peng, Shu-guang] Res Inst Hunan Tobacco Sci, Changsha 410007, Hunan, Peoples R China.;[Peng, Shu-guang] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Kang, Jing-he] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Wang, Zheng] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

摘要： Rosiglitazone (RG) is a well-known activator of peroxisome proliferator-activated receptor-gamma (PPARγ) and used to treat hyperglycemia and type 2 diabetes; however, its clinical application has been confounded by adverse side effects. Here, we assessed the roles of chlorogenic acid (CGA), a phenolic secondary metabolite found in many fruits and vegetables, on the differentiation and lipolysis of mouse 3T3-L1 preadipocytes. The results showed that CGA promoted differentiation in vitro according to oil red O staining and quantitative polymerase chain reaction assays. As a potential molecular mechanism, CGA downregulated mRNA levels of the adipocyte differentiation-inhibitor gene Pref1 and upregulated those of major adipogenic transcriptional factors (Cebpb and Srebp1). Additionally, CGA upregulated the expression of the differentiation-related transcriptional factor PPARγ2 at both the mRNA and protein levels. However, following CGA intervention, the accumulation of intracellular triacylglycerides following preadipocyte differentiation was significantly lower than that in the RG group. Consistent with this, our data indicated that CGA treatment significantly upregulated the expression of lipogenic pathway-related genes Plin and Srebp1 during the differentiation stage, although the influence of CGA was weaker than that of RG. Notably, CGA upregulated the expression of the lipolysis-related gene Hsl, whereas it did not increase the expression of the lipid synthesis-related gene Dgat1. These results demonstrated that CGA might function as a potential PPARγ agonist similar to RG; however, the impact of CGA on lipolysis in 3T3-L1 preadipocytes differed from that of RG. © 2018 Shu-guang Peng et al.

语种： 英文

作者： 王艳;林禹翔;康劲翮;王征

期刊： 营养学报,2018年40(4):353-359 ISSN：0512-7955

作者机构： 湖南农业大学生物科学技术学院, 长沙, 410128;湖南人文科技学院, 湖南省高校农药无害化应用重点实验室;[王艳; 林禹翔] 湖南省农田杂草防控技术与应用协同创新中心, 娄底, 417000;中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 长沙, 410125;[王艳] 湖南农业大学生物科学技术学院, 长沙, 410128 湖南人文科技学院, 湖南省高校农药无害化应用重点实验室

关键词： 玉竹多糖;脂代谢;肥胖

摘要： 目的研究玉竹多糖(POP)对小鼠脂肪代谢关键基因表达的影响,探讨POP干预肥胖形成的机制。方法采用C57BL/6健康清洁级小鼠,每天在喂饲高脂饲料的同时分别经口灌胃给予奥利司他(30 mg/kg·d)和玉竹多糖(400 mg/kg·d),期间跟踪小鼠能量摄入量和体质量变化,8 w实验结束后检测小鼠脂肪和肝脏湿重,测定血清血脂含量,采用qPCR技术检测小鼠肝脏脂代谢关键基因表达水平。结果与高脂模型组相比,玉竹多糖能明显抑制小鼠体质量增加,显著降低Lee's指数、身体质量指数、脂肪和肝脏系数、血清甘油三酯和低密度脂蛋白浓度(P <0.05);提高了血清高密度脂蛋白浓渡(P <0.05),对能量摄入量影响不明显。但显著减少肝脏脂肪合成相关基因脂蛋白脂酶、乙酰辅酶A羧化酶、脂肪酸合成酶、固醇调节元件结合蛋白-1c mRNA表达水平(P <0.05),上调脂肪分解相关基因激素敏感性甘油三酯脂肪酶mRNA表达(P <0.05),但对3-羟基-3-甲基戊二酰辅酶A还原酶mRNA表达影响不明显。结论玉竹多糖可以通过调节肝脏脂代谢相关基因的表达水平,促进脂肪氧化分解,抑制脂肪酸合成,抑制小鼠体质量的增加,从而有效预防小鼠肥胖的发生。

语种： 中文

作者： Yang, Huilin;Peng, Yuande;Shi, Yixue;Tian, Jianxiang;Wang, Juan;...

期刊： Ecotoxicology,2018年27(2):198-208 ISSN：0963-9292

通讯作者： Wang, Zhi;Lv, Zhiyue

作者机构： [Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin; Wang, Zhi; Shi, Yixue; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Xianjin; Xu, Xiang] Hunan Normal Univ, Coll Life Sci, Changsha 410006, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;[Lv, Zhiyue] S;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.

关键词： Cadmium;Transcriptomic;P. pseudoannulata;Cerebral ganglion

摘要： Cadmium (Cd) is a heavy metal that can cause irreversible toxicity to animals, and is an environmental pollutant in farmlands. Spiders are considered to be an excellent model for investigating the impacts of heavy metals on the environment. To date, the changes at the molecular level in the cerebral ganglia of spiders are poorly understood. Cd exposure leads to strong damage in the nervous system, such as apoptosis and necrosis of nerve cells, therefore we conducted a transcriptomic analysis of Pardosa pseudoannulata cerebral ganglia under Cd stress to profile differential gene expression (DGE). We obtained a total of 123,328 assembled unigenes, and 1441 Cd stress-associated DEGs between the Cd-treated and control groups. Expression profile analysis demonstrated that many genes involved in calcium signaling, cGMP—PKG signaling, tyrosine metabolism, phototransduction–fly, melanogenesis and isoquinoline alkaloid biosynthesis were up-regulated under Cd stress, whereas oxidative phosphorylation-related, nervous disease-associated, non-alcoholic fatty liver disease-associated, and ribosomal-associated genes were down-regulated. Here, we provide a comprehensive set of DEGs influenced by Cd stress, and heavy metal stress, and provide new information for elucidating the neurotoxic mechanisms of Cd stress in spiders. © 2018, The Author(s).

语种： 英文

作者： Wang, Yan;Fei, Yanquan;Liu, Lirui;Xiao, Yunhua;Pang, Yilin;...

期刊： International Journal of Molecular Sciences,2018年19(11) ISSN：1422-0067

通讯作者： Wang, Zheng;Kang, Jinhe

作者机构： [Fei, Yanquan; Pang, Yilin; Wang, Zheng; Xiao, Yunhua; Wang, Yan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Wang, Yan] Hunan Univ Humanities Sci & Technol, Coll Agr & Biotechnol, Loudi 417000, Peoples R China.;[Wang, Yan; Kang, Jinhe] Chinese Acad Sci, Inst Subtrop Agr,Minist Agr, CAS Key Lab Agroecol Proc Subtrop Reg,South Cent, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.;[Liu, Lirui] Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China.

通讯机构： [Wang, Zheng] H;[Kang, Jinhe] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Inst Subtrop Agr,Minist Agr, CAS Key Lab Agroecol Proc Subtrop Reg,South Cent, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.

关键词： Polygonatum odoratum;gut microbiota;high-fat diet;obesity;polysaccharides

摘要： Increasing evidence suggests that the gut microbiota plays vital roles in metabolic diseases. Polygonatum odoratum extract alleviates hyperglycemia and hyperlipidemia, but the underlying mechanism remains unclear. This study investigated the effects of P. odoratum polysaccharides (POPs) on high-fat diet (HFD)-induced obesity in rats and whether these effects were related to modulation of gut microbiota. POP treatment attenuated weight gain, fat accumulation, epididymal adipocyte size, liver triglycerides, and total liver cholesterol content in HFD-fed rats. POP administration also increased short-chain fatty acids (SCFAs), including isobutyric acid, butyric acid, and valeric acid. POP upregulated the expression of genes involved in adipocyte differentiation (Pparg, Cebpa, Cebpb) and lipolysis (Ppara, Atgl), and downregulated those related to lipid synthesis (Srebpf1, Fabp4, Fas), with corresponding changes in PPARγ and FABP4 protein expression. Finally, POP enhanced species richness and improved the gut microbiota community structure, reducing the relative abundances of Clostridium, Enterococcus, Coprobacillus, Lactococcus, and Sutterella. Principal coordinates analysis (PCoA) revealed a clear separation between HFD-fed rats and all other treatment groups. Correlation analysis identified negative and positive associations between obesity phenotypes and 28 POP-influenced operational taxonomic units (OTUs), including putative SCFA-producing bacteria. Our data suggest that POP supplementation may attenuate features of obesity in HFD-fed rats in association with the modulation of gut microbiota.

语种： 英文

作者： Wang, Juan;Wei, Baoyang;Peng, Yuande;Huang, Ting;Yang, Huilin;...

期刊： Environmental Science and Pollution Research,2018年25(34):34294-34305 ISSN：0944-1344

通讯作者： Wang, Zhi;Lv, Zhiyue

作者机构： [Peng, Xianjin; Wang, Zhi; Wang, Juan; Xu, Xiang] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Yang, Huilin; Wang, Zhi; Wei, Baoyang; Sun, Zhiying; Huang, Ting; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;[Lv, Zhiyue] S;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.

关键词： P. pseudoannulata;Cadmium;Detoxification;Immunity;Development

摘要： Cadmium (Cd) can be transferred and accumulated in spiders, posing a survival risk to them. To analyze potential biological damage caused by Cd accumulation and relevant detoxification strategies employed by spiders in response to Cd exposure, we conducted transcriptome analysis of the 5th instar spider P. pseudoannulata, a common spider species playing a vital role in natural pest control in agricultural fields of southern China. We obtained 92,778 unigenes with an average length of 1104 bp and identified 302, 655, and 424 differentially expressed genes (DEGs) in the spiders fed with Cd-containing fruit flies for 2, 5, and 8 days, respectively. Results showed that the body mass of Cd-containing P. pseudoannulata were reduced when compared with controls, presumably due to delayed maturation of tissues and organs. Meanwhile, functional analysis of DEGs indicated that Cd may have a negative effect on neural signal transduction and molt cycle of the spider. For defense strategies, detoxification enzymes like glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and P450, and typical proteins like heat shock protein and metallothionein were all differentially expressed in response to Cd stress. Besides, innate immune responses like toll-like receptor signaling pathways were also upregulated. Multiple critical Cd-responsive genes involved in biological damage, detoxification, and immune response were identified, providing referable foundation for further research on Cd toxicity to P. pseudoannulata. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Yang, Huilin;Peng, Yuande;Tian, Jianxiang;Wang, Juan;Hu, Jilin;...

期刊： Plant Cell Reports,2017年36(4):505-517 ISSN：0721-7714

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin; Wang, Zhi; Hu, Jilin; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： Spiders;Bt rice;Non-target effects;GM crops;Spider ecology

摘要： Since the birth of transgenic crops expressing Bacillus thuringiensis (Bt) toxin for pest control, the public debate regarding ecological and environmental risks as well as benefits of Bt crops has continued unabated. The impact of Bt crops, especially on non-target invertebrates, has received particular attention. In this review, we summarize and analyze evidences for non-target effects of Bt rice on spiders, major predators in rice fields. Bt rice has been genetically modified to express the Bt protein, which has been shown to be transferred and accumulate in spiders as part of their food chain. Moreover, the Bt protein exhibits unintended effects on the physiology of spiders and spreads to higher trophic levels. Spiders possess unique physiological and ecological characteristics, revealing traits of surrogate species, and are thus considered to be excellent non-target arthropod model systems for study of Bt protein impacts. Due to the complexities of Bt protein transfer and accumulation mechanisms, as well as the apparent lack of information about resulting physiological, biochemical, and ecological effects on spiders, we raise questions and provide recommendations for promising further research.

语种： 英文