作者机构：
[曹桂萍; 雷淑敏; 陈燕; 罗勇] School of Chemistry and Environmental Science, Xiangnan University, Chenzhou, 423000, China;Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, 410128, China;National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha, 410128, China;[李群] School of Pharmacy, Xiangnan University, Chenzhou, 423000, China;[温贝贝; 王坤波] Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, 410128, China, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha, 410128, China
通讯机构：
[Wang, K.] K;Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China
通讯机构：
[Guangcun Li] I;[Xingyao Xiong] H;Institute of Vegetables and Flowers, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Beijing 100081, China<&wdkj&>Hunan Provincial Engineering Research Center for Potatoes, Key Laboratory for Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China
摘要：
In order to elucidate the contributions of JA in orchestrating disease resistance in potato plants, the potato genotype ‘SD20’, which exhibits strong resistance against the highly virulent Phytophthora infestans isolate CN152, while infected by the super virulent isolate 2013-18-306, was treated with exogenous JA and then challenged by inoculation with 2013-18-306. The results showed that exogenously applied JA significantly delayed the onset and alleviated the symptoms of late blight, indicating exogenous JA could induce resistance to P. infestans in the early biotrophic stage of infection in ‘SD20’ plants. To further clarify the role of JA in the early defense response and identify key genes involved in JA signal transduction, gene expression profiling via RNA sequencing (RNA-seq) in ‘SD20’ plants treated with exogenously applied JA was performed. A total of 2 927 differentially expressed genes were specifically induced, the majority encoded transcription factors, protein kinases, secondary metabolites, defense enzymes and disease resistance related proteins. GO functional annotation and KEGG metabolic pathway analysis showed that exogenously applied JA rapidly induced the expression of genes related to immune response regulation, pathogen defense, and other biological processes, and stimulated endogenous JA synthesis and signal transduction, and the overall early pathogen defense response in ‘SD20’. These results provide useful information in understanding the JA's function involved in pathogen defense responses and a theoretical basis for the application of JA in potato production.
作者机构：
[李益; 金燕; 王萍; 盛玲; 麻明英; 邓子牛; 马先锋; 张凯] Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Ma, X.] E;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, China
作者机构：
[谢桂先; 彭建伟; 田昌; 荣湘民; 黄思怡] National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China;Rural Energy and Environment Agency, Ministry of Agriculture and Rural Affairs, Beijing, 100125, China;[周旋] Institute of Soil and Fertilizer, Hunan Academy of Agricultural Sciences, Changsha, 410125, China;[王英姿] College of Horticulture, Hunan Agricultural University, Changsha, 410128, China;[徐泽] Agricultural and Rural Bureau of Changsha County, Changsha, 410100, China
通讯机构：
[Wang, Y.-Z.] C;College of Horticulture, China
作者机构：
[牛丽; 李国萍; 卫艺炜; 刘巧灵; 朱海燕] Key Laboratory of Education for Tea Science, National Research Center of Engineering Technology for Utilization of Botanical Function Ingredients, Hunan Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Horticulture College, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Zhu, H.] K;Key Laboratory of Education for Tea Science, China
作者机构：
[杨艳芳; 邱德有] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China;[陆英; 唐其] College of Horticulture, Hunan Agricultural University, Changsha, 410128, China;[陈段芬] College of Horticulture, Hebei Agricultural University, Baoding, 071001, China;[吴长桥] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China;[蒋路园] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China, College of Horticulture, Hebei Agricultural University, Baoding, 071001, China
通讯机构：
[Yang, Y.-F.] S;State Key Laboratory of Tree Genetics and Breeding, China
作者机构：
[唐帅; 黄红梅; 薛帅; 章翼; 孔维政; 易自力] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China;Horticulture and Landscape College, Hunan Agricultural University, Changsha, 410128, China;National Center for Citrus Improvement, Changsha, 410128, China;[李大志] Horticulture and Landscape College, Hunan Agricultural University, Changsha, 410128, China, National Center for Citrus Improvement, Changsha, 410128, China
通讯机构：
[Yi, Z.] C;College of Bioscience and Biotechnology, China
作者机构：
湖南农业大学, 茶学教育部重点实验室, 长沙, 410128;湖南农业大学, 国家植物功能成分利用工程技术研究中心, 长沙, 410128;中茶湖南安化第一茶厂有限公司, 安化, 413500;湖南农业大学, 湖南省植物功能成分利用省部共建协同创新中心, 长沙, 410128;[陈崇俊; 冉莉莎; 刘宝贵; 唐倩] Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, 410128, China, National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Zhu, M.; Fu, D.; Wang, K.] K;Key Laboratory of Ministry of Education for Tea Science, China
摘要：
Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China. To increase the availability of DNA markers in B. rapa, we developed insertion-deletion（In Del） markers utilizing high-resolution melting（HRM） curve analysis. We designed primers for 252 In Dels（≥ 3 bp） evenly distributed in the genome and tested gene polymorphisms with eight accessions. In total, 208 markers were speciically ampliied, and 148 In Dels with polymorphism were genotyped successfully using HRM. We further analyzed the correlation with In Del size,GC number, and predicted the difference in Tmvalues（ Tm） using 208 markers with speciic ampliication. We found that the success rate of In Del markers was correlated with the GC number of In Del and the predicted-Tm, but not clearly correlated with the length of In Del. When the GC number within In Del was ≥ 8, the successful rate exceeded 90.0%. When the predicted-Tmreached 0.5 °C, the success rate was greater than 90.0%, and when it was ≥ 0.6 °C, the rate climbed to 100.0%, indicating their role as the optimal parameter for successful development of an applicable In Del marker. The polymorphic In Del markers can be easily genotyped using HRM. They are of great value in genetic analysis,construction of linkage map, and molecular marker-assisted selection in B. rapa.
摘要：
Genes containing GTPEFTU domain mainly express elongation factors（EF）, Small GTPases, and GTP-binding proteins, which are closely related to protein synthesis, extension and ATP synthesis. In this study, we identified 39 genes containing GTPEFTU domains from peppers.The evolutionary trees constructed from capsicum, Arabidopsis, rice, and tomato are mainly divided into 7 subfamilies. Using PacBio（Pacific Biosciences） sequencing and assembly data, we extracted these 39 gene sequences, from which 25 genes had alternative splicing. Particularly, the Capana08 g000545 had 16 alternative splicing processes. Accordingly, we performed promoter sequence analysis, subcellular location prediction,the expression analysis of different tissues and periods, and also the GO（Gene ontology） analysis of co-expressed genes. Lastly we did the qRTPCR analysis in 5 stages of pepper fruit development. These analyses revealed important structural and functional information for the identified39 genes that contain GTPEFTU domains, providing important references for further follow-up experiments to verify the genes function on plants or their unique roles in peppers.
