微生物发酵对木薯渣营养成分的影响
作者：
蒋慧姣;李净;彭辉平;倪姮佳;方俊;...
期刊：
微生物学通报 ,2021年48(02):407-413 ISSN：0253-2654
作者机构：
[彭辉平; 倪姮佳; 孔祥峰; 李净] 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室动物营养生理与代谢过程湖南省重点实验室;[方俊] 湖南农业大学生物科学技术学院湖南省猪场废弃物无害化处理与资源化利用工程研究中心;[蒋慧姣] 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室动物营养生理与代谢过程湖南省重点实验室<&wdkj&>湖南农业大学生物科学技术学院湖南省猪场废弃物无害化处理与资源化利用工程研究中心
关键词：
微生物发酵;木薯渣;营养成分;饲料原料
摘要：
【背景】将木薯渣作为饲料资源进行开发和利用能够减轻环境污染,实现资源就地转化,已成为国内外研究热点。微生物发酵可降低木薯渣的粗纤维含量,改善适口性,提高饲料转化率。【目的】筛选对木薯渣发酵效果较好的微生物发酵剂及其发酵时长。【方法】试验选用A (芽孢菌+乳酸菌+酿酒酵母菌)、B (戊糖片球菌+酿酒酵母菌)和C (植物乳杆菌+枯草芽孢杆菌+酿酒酵母菌)这3种复合微生物发酵剂与新鲜木薯渣混合,随机分为7组,每组5个重复,分别于发酵的第0、1、3、7、14、28和56天取样,测定其常规营养成分。【结果】与发酵前相比,发酵剂A和B可显著增加木薯渣的粗灰分含量(P<0.05),发酵剂C可显著增加木薯渣的粗蛋白质含量(P<0.05);3种发酵剂均可显著降低木薯渣的中性洗涤纤维含量、显著增加粗脂肪含量(P<0.05)。【结论】微生物发酵可有效改善木薯渣的营养价值,其中利用发酵剂C进行短期发酵对其营养价值,尤其是粗蛋白质和粗脂肪含量的提高最明显。
语种：
中文
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Thermochemical liquefaction of cattle manure using ethanol as solvent: Effects of temperature on bio-oil yields and chemical compositions
作者：
Fang, Jun;Liu, Zhuangzhuang;Luan, Hui;Liu, Fen;Yuan, Xingzhong;...
期刊：
Renewable Energy ,2021年167:32-41 ISSN：0960-1481
通讯作者：
Xiao, Zhihua
作者机构：
[Xiao, Zhihua; Liu, Zhuangzhuang; Luan, Hui; Liu, Fen; Long, Shundong; Wang, Andong; Ma, Yong; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Zhihua; Liu, Zhuangzhuang; Luan, Hui; Liu, Fen; Long, Shundong; Wang, Andong; Ma, Yong; Fang, Jun] Hunan Engn Lab Pollut Control & Waste Utilizat Sw, Changsha 410128, Peoples R China.;[Xiao, Zhihua; Liu, Zhuangzhuang; Luan, Hui; Liu, Fen; Long, Shundong; Wang, Andong; Ma, Yong; Fang, Jun] Key Lab Rural Ecosyst Hlth Dongting Lake Area Hun, Changsha 410128, Peoples R China.;[Yuan, Xingzhong] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Peoples R China.
通讯机构：
[Xiao, Zhihua] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Cattle manure;Thermochemical liquefaction;Ethanol;Bio-oil
摘要：
Cattle manure was converted into bio-oil via suband super-critical liquefaction with ethanol as the solvent. The effects of various reaction parameters, including the reaction temperature (T, 180-300 degrees C), solid-liquid ratio (S-1, 5-15%), and solvent filling ratios (S-2, 10-30%) on the yield of bio-oil (OCM) and residue (RCM) from liquefaction of cattle manure were studied. The yield of bio-oil was positively correlated to reaction temperature suggesting higher reaction temperature could promote the fragmentation of the polymers transformed into a liquid oil-rich phase and the maximum OCM relative yield (32.14 +/- 0.19%) was obtained at 300 degrees C. OCM had HHV of 25.63-33.41 MJ/kg and LHV of 23.85-31.39 MJ/ kg, respectively, suggesting their potential for use as a renewable energy to replace fossil energy. The GC-MS analyses demonstrated that the major compounds in OCM were esters, hydrocarbons, alcohols, phenolic compounds, nitrogenous and bromine components, the compositions of OCM was similar to that of biodiesel. The presence of ester compounds in OCM which was attributed to the degradation of cellulose and hemicelluloses. Carbon-normal paraffin gram (C-NP) analysis indicated that C-18 and C-20 species comprised the bulk of the hydrocarbon compounds from OCM. (c) 2020 Elsevier Ltd. All rights reserved.
语种：
英文
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Dietary polyphenols in lipid metabolism: A role of gut microbiome
作者：
Ma, Jie;Zheng, Yongmin;Tang, Wenjie;Yan, Wenxin;Nie, Houfu;...
期刊：
动物营养(英文) ,2020年6(4):404-409 ISSN：2405-6545
通讯作者：
Liu, Gang
作者机构：
[Liu, Gang; Yan, Wenxin; Zheng, Yongmin; Tang, Wenjie; Ma, Jie; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Anim Sci & Technol, Hunan Prov Engn Res Ctr Appl Microbial Resources, Changsha, Hunan, Peoples R China.;[Tang, Wenjie] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.;[Tang, Wenjie] Sichuan Acad Anim Sci, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu, Peoples R China.;[Nie, Houfu] Agr & Rural Off Chunkou Town, Liuyang, Hunan, Peoples R China.
通讯机构：
[Liu, Gang] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Anim Sci & Technol, Hunan Prov Engn Res Ctr Appl Microbial Resources, Changsha, Hunan, Peoples R China.
关键词：
Polyphenols;Lipid metabolism;Obesity;Gut microbiota
摘要：
Polyphenols are a class of non-essential phytonutrients, which are abundant in fruits and vegetables. Dietary polyphenols or foods rich in polyphenols are widely recommended for metabolic health. Indeed, polyphenols (i.e., catechins, resveratrol, and curcumin) are increasingly recognized as a regulator of lipid metabolism in host. The mechanisms, at least in part, may be highly associated with gut microbiome. This review mainly discussed the beneficial effects of dietary polyphenols on lipid metabolism. The potential mechanisms of gut microbiome are focused on the effect of dietary polyphenols on gut microbiota compositions and how gut microbiota affect polyphenol metabolism. Together, dietary polyphenols may be a useful nutritional strategy for manipulation of lipid metabolism or obesity care. © 2020 Chinese Association of Animal Science and Veterinary Medicine
语种：
英文
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防御素对肠道炎症反应和微生物的调节机制
作者：
颜文新;刘刚;方俊
期刊：
饲料工业 ,2020年41(11):61-64 ISSN：1001-991X
作者机构：
[刘刚] 湖南农业大学生物科学技术学院,湖南长沙410128;[刘刚] 中国科学院亚热带农业生态研究所,湖南长沙410125;[颜文新; 方俊] 湖南农业大学
关键词：
仔猪;防御素;微生物;炎症反应;潘氏细胞
摘要：
仔猪肠道易受病原等侵袭,容易发生炎性反应。防御素是先天免疫的主要效应物,在宿主免疫系统中起着重要的作用,其重要性已被广泛认可。防御素通过介导微生物调节肠道炎症而成为抗炎治疗潜在的新靶点。文章综述了防御素在肠道炎症反应中的作用,并对防御素调控仔猪肠道炎性反应的机制进行了讨论。
语种：
中文
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The migration, transformation, and risk assessment of heavy metals in residue and bio-oil obtained by the liquefaction of pig manure
作者：
Luan, Hui;Liu, Fen;Long, Shundong;Liu, Zhuangzhuang;Qi, Yanting;...
期刊：
Environmental Science and Pollution Research ,2020年28(12):15055-15069 ISSN：0944-1344
通讯作者：
Xiao, Zhihua;Fang, Jun
作者机构：
[Xiao, ZH; Fang, Jun; Xiao, Zhihua; Luan, Hui; Liu, Zhuangzhuang; Liu, Fen; Long, Shundong] Hunan Agr Univ, Hunan Engn Lab Pollut Control & Waste Utilizat Sw, Changsha 410128, Peoples R China.;[Luan, Hui; Liu, Zhuangzhuang; Liu, Fen; Long, Shundong; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Qi, Yanting; Xiao, Zhihua] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.;[Qi, Yanting; Xiao, Zhihua] Key Lab Rural Ecosyst Hlth Dongting Lake Area Hun, Changsha 410128, Peoples R China.
通讯机构：
[Xiao, ZH; Fang, J; Fang, Jun; Xiao, Zhihua] H;[Xiao, Zhihua] K;Hunan Agr Univ, Hunan Engn Lab Pollut Control & Waste Utilizat Sw, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
关键词：
Biofuel;Chemical speciation;Livestock excrement;Pollutant;Thermal treatment;Toxicity;X-ray diffraction
摘要：
The total contents and chemical speciation analysis of Zn, Cu, Pb, Cr, Mn, Ni, Cd, and As in pig manure (PM), liquefaction residues (LRs), and bio-oils (BOs) derived from PM by liquefaction with ethanol as a solvent at 180–300 °C were thoroughly investigated in this study. The environment risk assessment, leachability, and bioavailability of heavy metals in PM and LRs were studied. The results showed that more than 75% of heavy metals remained in LRs. The total contents of heavy metals in LRs were markedly elevated, but those in BOs gradually decreased with the increase in liquefaction temperature. Moreover, the acid soluble/exchangeable fraction and reducible fraction (F1 + F2) of heavy metals in LRs and BOs was significantly reduced, while oxidizable fraction and stable fraction (F3 + F4) desirably increased after liquefaction. Furthermore, the potential risk of heavy metals in LRs was decreased in comparison to that in PM, but the risk of Pb, Mn, and As had not been obviously reduced; therefore, the LRs from the liquefaction of PM should be pretreated before recycling. Temperatures from 220 to 260 °C were the optimum conditions for disposing of PM by liquefaction with ethanol. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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生态学专业土壤生态学实验教学改革重点——以湖南农业大学为例
作者：
刘芬;方俊;肖智华
期刊：
科教导刊 ,2020年(25):47-48 ISSN：1674-6813
作者机构：
[刘芬; 方俊] 湖南农业大学生物科学技术学院 湖南·长沙 410128;[刘芬; 方俊] 湖南省猪场废弃物无害化处理与资源化利用工程技术研究中心 湖南·长沙 410128;湖南农业大学资源与环境学院 湖南·长沙 410128;[肖智华] 湖南农业大学
关键词：
生态学专业;土壤生态学;实验课程;教学改革
摘要：
土壤生态学是生态学的新兴分支学科,并逐渐成为了生态学重要的组成部分.针对目前生态学专业土壤生态学实验教学实际中存在的主要问题,对课时设置、实验内容、教学方法以及考核体系提出了具体的改革意见,以期为生态学***创新创业人才的培养奠定基础.
语种：
中文
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Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis
作者：
Zhang, Yumei;Guo, Songge;Xie, Chunyan* ;Fang, Jun*
期刊：
BioMed Research International ,2020年2020 ISSN：2314-6133
通讯作者：
Xie, Chunyan;Fang, Jun
作者机构：
[Xie, Chunyan; Fang, Jun; Zhang, Yumei; Guo, Songge] Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xie, CY; Fang, J] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.
摘要：
Pyrimidine nucleoside uridine plays a critical role in maintaining cellular function and energy metabolism. In addition to its role in nucleoside synthesis, uridine and its derivatives contribute to reduction of cytotoxicity and suppression of drug-induced hepatic steatosis. Uridine is mostly present in blood and cerebrospinal fluid, where it contributes to the maintenance of basic cellular functions affected by UPase enzyme activity, feeding habits, and ATP depletion. Uridine metabolism depends on three stages: De novo synthesis, salvage synthesis pathway and catabolism, and homeostasis, which is tightly relating to glucose homeostasis and lipid and amino acid metabolism. This review is devoted to uridine metabolism and its role in glucose, lipid, and amino acid homeostasis. © 2020 Yumei Zhang et al.
语种：
英文
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Modulatory Mechanisms of the NLRP3 Inflammasomes in Diabetes.
作者：
Ding, Sujuan;Xu, Sheng;Ma, Yong;Liu, Gang* ;Jang, Hongmei* ;...
期刊：
Biomolecules ,2019年9(12) ISSN：2218-273X
通讯作者：
Liu, Gang;Jang, Hongmei;Fang, Jun
作者机构：
[Liu, Gang; Ding, Sujuan; Ma, Yong; Jang, Hongmei; Fang, Jun; Liu, G] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xu, Sheng] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China.;[Liu, Gang] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Natl Engn Lab Pollut Control & Waste Utilizat Liv, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Liu, G; Jang, HM; Fang, J] H;[Liu, Gang] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Natl Engn Lab Pollut Control & Waste Utilizat Liv, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Changsha 410125, Hunan, Peoples R China.
关键词：
Glucose tolerance;NLRP3;diabetes;gut microbes;insulin resistance
摘要：
The inflammasome is a multiprotein complex that acts to enhance inflammatory responses by promoting the production and secretion of key cytokines. The best-known inflammasome is the NLRP3 (nucleotide-binding oligomerization domain-like receptor [NLR] family pyrin domain-containing 3) inflammasome. The evidence has shown that the NLRP3 inflammasome, IL-1beta, thioredoxin-interacting protein (TXNIP), and pyroptosis play vital roles in the development of diabetes. This review summarizes the regulation of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) by NLRP3 via modulation of glucose tolerance, insulin resistance, inflammation, and apoptosis mediated by endoplasmic reticulum stress in adipose tissue. Moreover, NLRP3 participates in intestinal homeostasis and inflammatory conditions, and NLRP3-deficient mice experience intestinal lesions. The diversity of an individual's gut microbiome and the resultant microbial metabolites determines the extent of their involvement in the physiological and pathological mechanisms within the gut. As such, further study of the interaction between the NLRP3 inflammasome and the complex intestinal environment in disease development is warranted to discover novel therapies for the treatment of diabetes.
语种：
英文
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Impact of sulfur-containing amino acids on the plasma metabolomics and intestinal microflora of the sow in late pregnancy
作者：
Ding, Sujuan;Azad, Md Abul Kalam;Fang, Jun;Zhou, Xihong;Xu, Kang;...
期刊：
FOOD & FUNCTION ,2019年10(9):5910-5921 ISSN：2042-6496
通讯作者：
Liu, Gang
作者机构：
[Zhou, Xihong; Liu, Gang; Azad, Md Abul Kalam; Ding, Sujuan; Yin, Yutong; Xu, Kang] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Natl Engn Lab Pollut Control & Waste Utilizat Liv, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Changsha 410125, Hunan, Peoples R China.;[Liu, Gang; Ding, Sujuan; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Azad, Md Abul Kalam] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Zhou, Xihong; Liu, Gang; Yin, Yutong] Shaoguan Jinpingguo Feeds Co Ltd, Academician Workstn, Guangzhou 512027, Guangdong, Peoples R China.
通讯机构：
[Liu, Gang] C;[Liu, Gang] H;[Liu, Gang] S;Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Natl Engn Lab Pollut Control & Waste Utilizat Liv, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
摘要：
Sulfur-containing amino acids (SAAs) such as methionine (Met) and cysteine (Cys) are involved in the control of oxidative states and may serve as mediators of metabolism and cellular functions. The purpose of this study was to evaluate the influence of different Met/Cys ratios (46% Met, 51% Met, 56% Met, and 61% Met, with Met accounting for 0.78% of the total SAAs and Cys for the remainder; see Methods section for details) on the plasma metabolomics, intestinal microflora of the sow in late pregnancy and survival rate of piglets. The results demonstrated that the 51% Met group increased the survival rate of piglets and improved the level of low-density lipoprotein, albumin, and phosphorus of serum in comparison with the 56% Met group. The resulting plasma metabolomic profile indicated that the 51% Met group had enhanced levels of β-alanine, uracil, hydrouracil, phosphoserine, vitamin D3, and α-tocotrienol compared with the 46% Met group. Moreover, the ratio of Bacteroidetes and Firmicutes was increased in the 51% Met and 62% Met groups in relation to the 56% Met group. In addition, Pearson's correlation analysis found that there were negative correlations between vitamin D3 and Ruminococcaceae-UCG-002 and between α-tocotrienol and Ruminococcaceae-UCG-002; there was a positive correlation between allantoin and Parabacteroides spp. These results suggested that 51% Met is the optimal Met/Cys ratio for sows in late pregnancy, because it increased the survival percentage of piglets and enhanced the increase in serum metabolites required for optimal sow health during late pregnancy. © 2019 The Royal Society of Chemistry.
语种：
英文
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IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation
作者：
Ma, Yong;Jiang, Hongmei* ;Fang, Jun* ;Liu, Gang
期刊：
BioMed Research International ,2019年2019 ISSN：2314-6133
通讯作者：
Jiang, Hongmei;Fang, Jun
作者机构：
[Liu, Gang; Jiang, Hongmei; Fang, Jun; Ma, Yong] Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Liu, Gang] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Anim Nutr Physiol & Metab Proc, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Jiang, HM; Fang, J] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
摘要：
Background and Objective. Bioactive peptides exert great influence in animals and human health by targeting gastrointestinal tracts. The colitis model of mice was induced by dextran sulfate sodium (DSS). Thirty-two 8-week-old mice weighing 23 g on average were randomly assigned to four groups of 8 each: Mice fed basal diet (CON), mice fed basal diet with 5% DSS (DSS), mice fed 0.03% IRW with 5% DSS (IRW-DSS), and mice fed 0.03% IRW with 5% DSS (IQW-DSS). After an adaptation period of 3 days, on day 8, all mice were slaughtered. Serum samples were collected to determine the level of amino acids; colonic tissue was quick-frozen for the determination of gene expression. Methods. The aim of this study was to assess the ability of two kinds of peptides (IRW and IQW) to repair intestinal inflammatory in the DSS-induced model in accordance with serum amino acids and intestinal inflammatory factors. Results. The results demonstrated that the addition of IRW and IQW had a mitigating effect on DSS-induced intestinal inflammation. The level of Asp decreased in the serum of mice supplemented with IRW-DSS (P<0.05), and IQW enhanced the level of Leu, but lowered the level of Ser (P<0.05). IQW and IRW addition reduced the level of TNF-α and IL-17 (P<0.05). No other significant effects were observed. Conclusions. The present study demonstrated that intracolic administration of IRW and IQW might be a novel option for preventing inflammatory bowel disease via regulating the level of serum amino acid and enhancing the intestinal immune defense. © 2019 Yong Ma et al.
语种：
英文
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Lactobacillus brevis Alleviates DSS-Induced Colitis by Reprograming Intestinal Microbiota and Influencing Serum Metabolome in Murine Model
作者：
Ding, Sujuan;Ma, Yong;Liu, Gang* ;Yan, Wenxin;Jiang, Hongmei;...
期刊：
Frontiers in Physiology ,2019年10:461824 ISSN：1664-042X
通讯作者：
Liu, Gang;Fang, Jun
作者机构：
[Liu, Gang; Yan, Wenxin; Ding, Sujuan; Liu, G; Fang, Jun; Ma, Yong; Jiang, Hongmei] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Liu, Gang] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, G; Fang, J] H;[Liu, Gang] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Changsha, Hunan, Peoples R China.
关键词：
Colitis;Murine (mouse);Lactobacillus brevis;intestinal microbiota;Metabolome
摘要：
The aim of this study was to examine the effects of Lactobacillus brevis on the microbial community and serum metabolome in colitis induced by dextran sulfate sodium (DSS). ICR mice were randomly distributed into three treatment groups: (i) L. brevis treatment alone (control), (ii) DSS administration alone, and (iii) treatment with L. brevis and DSS. Our results demonstrate that L. brevis treatment significantly alleviated DSS-induced body weight loss and colon inflammation. In addition, LC-MS analysis of serum metabolites revealed that L. brevis treatment increased the serum level of metabolites against inflammatory responses or oxidative stressors caused by DSS in the murine model. By detecting colonic microbiota, L. brevis increased colonic microbial diversity after challenging with DSS, and increased the relative abundance of Alloprevotella at genus, but Bacteroidales was reduced (P < 0.05). These result indicated that L. brevis could lower the severity of colitis induced by DSS via improving reprogramming the serum metabolome and intestinal microbiota. These findings suggest that the probiotic L. brevis may prevent tissue damage from colitis.
语种：
英文
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MicroRNA determines the fate of intestinal epithelial cell differentiation and regulates intestinal diseases
作者：
Ding, Sujuan;Liu, Gang;Jiang, Hongmei* ;Fang, Jun*
期刊：
CURRENT PROTEIN & PEPTIDE SCIENCE ,2019年20(7):666-673 ISSN：1389-2037
通讯作者：
Jiang, Hongmei;Fang, Jun
作者机构：
[Liu, Gang; Jiang, Hongmei; Fang, Jun; Ding, Sujuan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Liu, Gang; Ding, Sujuan] Chinese Acad Sci, Inst Subtrop Agr, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Key Lab Agroecol Proc Subtrop Reg,Natl Engn Lab P, Changsha, Hunan, Peoples R China.;[Ding, Sujuan] Academician Workstn Hunan Baodong Farming Co Ltd, Changsha 422001, Hunan, Peoples R China.
通讯机构：
[Jiang, HM; Fang, J] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
miRNA;intestinal stem cells;differentiation;cancer;inflammatory bowel disease;mucosal inflammation.
摘要：
The rapid self-renewal of intestinal epithelial cells enhances intestinal function, promotes the nutritional needs of animals and strengthens intestinal barrier function to resist the invasion of foreign pathogens. MicroRNAs (miRNAs) are a class of short-chain, non-coding RNAs that regulate stem cell proliferation and differentiation by down-regulating hundreds of conserved target genes after transcription via seed pairing to the 3' untranslated regions. Numerous studies have shown that miRNAs can improve intestinal function by participating in the proliferation and differentiation of different cell populations in the intestine. In addition, miRNAs also contribute to disease regulation and therefore not only play a vital role in the gastrointestinal disease management but also act as blood or tissue biomarkers of disease. As changes to the levels of miRNAs can change cell fates, miRNA-mediated gene regulation can be used to update therapeutic strategies and approaches to disease treatment.
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英文
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专业认知度调查及专业导论课程的改革探讨——以湖南农业大学生态学专业为例
作者：
梁运姗;孙文笑;曾洪;张学文;方俊;...
期刊：
科教文汇(下旬刊) ,2019年(24):64-67 ISSN：1672-7894
作者机构：
湖南农业大学生物科学技术学院洞庭湖区农村生态系统健康湖南省重点实验室 湖南·长沙 410128;湖南省猪场废弃物无害化处理与资源化利用工程研究中心 湖南·长沙 410128
关键词：
专业认知度;专业导论课程;教学改革;生态学
摘要：
专业导论课的设置能帮助学生更好地认知自己所学专业,从而协助学生制定合理的职业生涯规划。本文以湖南农业大学生态学专业的学生为调查对象,对不同年级学生的专业认知度和专业发展规划进行了分析,并对目前专业导论课程的存在问题及改革策略进行了探讨,拟为提高学生的专业认知度和改良专业导论课的教学方式提供指导。
语种：
中文
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Antimicrobial activity of anthocyanins and catechins against foodborne pathogens Escherichia coli and Salmonella
作者：
Ma, Yong;Ding, Sujuan;Fei, Yanquan;Liu, Gang* ;Jang, Hongmei* ;...
期刊：
Food Control ,2019年106:106712 ISSN：0956-7135
通讯作者：
Liu, Gang;Jang, Hongmei;Fang, Jun
作者机构：
[Fei, Yanquan; Liu, Gang; Ding, Sujuan; Ma, Yong; Jang, Hongmei; Fang, Jun; Liu, G] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Liu, Gang] Chinese Acad Sci, Inst Subtrop Agr, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg,Natl Engn L, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Liu, G; Jang, HM; Fang, J] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Anthocyanins;Catechins;Escherichia coli;foodborne diseases;Gut microbiota;Salmonella
摘要：
The main foodborne pathogens are Norovirus, Enteric parasites, bacteria and fungi. Foodborne diseases can lead to high morbidity and mortality and result in huge economic losses. Some foodborne diseases are associated with antibiotic resistance, which is a significant problem throughout the world. To reduce the harm caused by antibiotics, it is important to find alternatives so that food is not infected with foodborne pathogens. Anthocyanins and catechins, two species of plant polyphenols with strong antioxidant properties, offer a promising new approach to preventing foodborne disease. Recent studies have shown that these compounds are good growth inhibitors. In this review, we focus on the antibacterial properties of anthocyanins and catechins as antibiotic substitutes. The minimum inhibitory concentration (MIC) of anthocyanins against E. coli and Salmonella is 10–400 mg/ml. The MIC of catechins against E. coli and Salmonella is 6–50 mg/ml. Anthocyanins and catechins can also significantly reduce the abundance of pathogenic bacteria that produce toxins in the host including Desulfovibrio sp. and Enterococcus. At the same time, we summarize the antibacterial mechanism of Anthocyanins and catechins on Salmonella and Escherichia coli. Anthocyanins and catechins can increase the abundance of probiotics such as Akkermansia, Bifidobacteria, and increase the ratio of thick-walled bacteria to Bacteroides. Finally, it seems that Anthocyanins and catechins can regulate the composition of intestinal microbes to improve intestinal immunity and promote intestinal health, thereby controlling foodborne. © 2019 Elsevier Ltd
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英文
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Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge
作者：
Ding, Sujuan;Wang, Yongwei;Yan, Wenxin;Li, Aike;Jiang, Hongmei* ;...
期刊：
PLOS ONE ,2019年14(6):e0212079 ISSN：1932-6203
通讯作者：
Jiang, Hongmei;Fang, Jun
作者机构：
[Jiang, Hongmei; Fang, Jun; Yan, Wenxin; Ding, Sujuan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Wang, Yongwei; Ding, Sujuan; Li, Aike] Acad Natl Food & Strateg Reserves Adm, Beijing, Peoples R China.;[Ding, Sujuan] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.
通讯机构：
[Jiang, HM; Fang, J] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
摘要：
One-day-old broilers were randomly allocated to five treatment groups: basal diet and orally administered sterile saline (negative control, n-control); basal diet challenged with E. coli O78 (positive control, p-control); basal diet supplemented with 1×108 CFU/kg L. plantarum 15-1 and challenged with E. coli O78 (LP); basal diet supplemented with 5 g/kg fructooligosaccharides (FOS) and challenged with E. coli O78 (FOS); and basal diet supplemented with both L. plantarum 15-1 and FOS and challenged with E. coli O78 (LP+FOS). The broilers in the LP, FOS, and LP+FOS groups displayed a decrease of crypt depth at day 14 compared with the control groups. Furthermore, at days 14 and 21, the broilers in the LP group exhibited reduced serum levels of diamine oxidase (DAO) compared with the p-control group (p<0.05), and the broilers in the LP+FOS group showed increased serum concentrations of IgA and IgG relative to both control groups and decreased DAO levels compared with the p-control group (p<0.05). Moreover, the LP group displayed higher levels of acetic acid and total short-chain fatty acids (SCFAs) compared with the p-control group at day 14 (p<0.05), and the FOS group showed higher levels of valeric acid and total SCFAs at day 21 (p<0.05). The LP+FOS group also displayed a higher level of butyric acid at day 14 (p<0.05). In conclusion, dietary supplementation with FOS improved the growth performance, while supplementation with L. plantarum 15-1 and FOS improved intestinal health by increasing the levels of SCFAs and mitigating the damage caused by E. coli O78, thus preventing intestinal damage and enhancing the immune response. © 2019 Ding et al.
语种：
英文
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浒苔多糖对ETEC感染小鼠肠道黏膜损伤的保护作用及机制研究
作者：
解影;马勇;谢春艳;王海华;台文静;...
期刊：
中国饲料 ,2019年(23):64-68 ISSN：1004-3314
作者机构：
湖南农业大学生物科学技术学院湖南省猪场废弃物无害化处理与资源化利用工程研究中心;[王海华; 台文静] 青岛海大生物集团有限公司;[马勇; 解影; 方俊; 谢春艳] 湖南农业大学
关键词：
浒苔多糖;小鼠;黏膜损伤;肠道屏障
摘要：
为探讨浒苔多糖(EP)对产肠毒素大肠杆菌(ETEC)感染小鼠肠道黏膜的保护作用及最适添加剂量,试验分为空白对照组、ETEC模型组、300 mg/L EP+ETEC组、600 mg/L EP+ETEC组和1000 mg/L EP+ETEC组.所有小鼠饲喂维持饲粮,对照组饲喂饮用水,EP试验组在饮用水中添加相应剂量浒苔多糖.造模采用灌胃ETEC方法,并对小鼠血清炎性因子及空肠相关蛋白丰度进行检测.结果 表明:添加600 mg/L剂量的浒苔多糖使感染后小鼠血清中TNF-α及IL-6水平显著降低30.22％、56.70％(P＜ 0.05),Claudin-1在小鼠空肠中的表达丰度显著上调80％(P＜0.05).结合空肠NF-κB信号通路检测结果表明,浒苔多糖可缓解ETEC感染导致的小鼠肠道黏膜损伤,且作用机制与其对NF-κB信号通路的影响相关.
语种：
中文
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生态学背景下的“环境影响评价”课程教学方案研究
作者：
梁运姗;雷龙;吴爱平;邹冬生;方俊
期刊：
科教导刊 ,2019年(2):92-94 ISSN：1674-6813
作者机构：
湖南农业大学生物科学技术学院,洞庭湖区农村生态系统健康湖南省重点实验室 湖南·长沙 410128;湖南省猪场废弃物无害化处理与资源化利用工程研究中心 湖南·长沙 410128;[雷龙; 梁运姗; 邹冬生; 方俊; 吴爱平] 湖南农业大学
关键词：
生态学;环境影响评价;教学方案
摘要：
基于目前很多高职院校以及高等院校对于环境类专业学生开设的"环境影响评价"课程的建设与探讨相当多,而针对生态学专业学生的该课程建设探讨较少,笔者针对湖南农业大学的生态学专业本科生培养,进行该课程的教学方案研究,从课程建设的必要性、目标、内容、教学方法与手段等方面提出相应措施,以供生态学专业培养与教学工作的借鉴参考,并以期培养出高素质的生态学人才。
语种：
中文
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The impact of different levels of cysteine on the plasma metabolomics and intestinal microflora of sows from late pregnancy to lactation.
作者：
Ding, Sujuan;Fang, Jun;Liu, Gang* ;Veeramuthu, Duraipandiyan;Naif Abdullah, Al-Dhabi;...
期刊：
FOOD & FUNCTION ,2019年10(2):691-702 ISSN：2042-6496
通讯作者：
Liu, Gang
作者机构：
[Liu, Gang; Ding, Sujuan; Yin, Yulong] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr,Natl Engn Lab Pollut Control & W, Changsha 410125, Hunan, Peoples R China.;[Liu, Gang; Ding, Sujuan; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Liu, Gang] Hunan Baodong Farming Co Ltd, Academician Workstn, Changsha 422001, Hunan, Peoples R China.;[Naif Abdullah, Al-Dhabi; Veeramuthu, Duraipandiyan] King Saud Univ, Dept Bot & Microbiol, Coll Sci, POB 2455, Riyadh 11451, Saudi Arabia.
通讯机构：
[Liu, Gang] C;[Liu, Gang] H;Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr,Natl Engn Lab Pollut Control & W, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Baodong Farming Co Ltd, Academician Workstn, Changsha 422001, Hunan, Peoples R China.
摘要：
Cysteine (Cys) is a semi-essential amino acid that is synthesized from methionine in mammals and affects their physiological state. This study aimed at investigating the effects of different Cys levels on the birth weight and survival rate of piglets as well as the plasma biochemical parameters, intestinal microbial diversity, and plasma metabolome of sows during late pregnancy. The results showed that 0.4% Cys supplementation increased the birth weight of piglets and decreased the calcium, triglyceride, and bilirubin levels in sows, whereas 0.5% Cys supplementation reduced the gamma-glutamyl transpeptidase levels and increased the serum glucose levels in sows at farrowing. Intestinal microbial analysis demonstrated that 0.4% Cys supplementation increased the diversity of fecal and intestinal microbiota compared with 0.5% Cys supplementation. In addition, plasma metabolomics identified 11 differential metabolites among the 0.4% Cys, 0.5% Cys, and control (basal diet) groups. The serum hypotaurine levels in sows increased by 0.4% and 0.5% Cys supplementation, and the serum acetylcysteine levels increased by 0.5% Cys supplementation; however, the differences in hypotaurine and acetylcysteine levels between the 0.4% and 0.5% Cys groups were not significant. Furthermore, Pearson analysis revealed a positive correlation between the hypotaurine levels and the abundance of Lactobacillus or Pseudobutyrivibrio and a negative correlation between the acetylcysteine levels and the abundance of Ruminococcaceae-UCG-014. Overall, the results indicated that 0.4% Cys supplementation increased the birth weight of piglets, increased the differential metabolites beneficial for combating antioxidative stress in embryos enhancing the intestinal microbial abundance in sows, and increased the diversity of fecal microbiota in sows. Thus, these findings suggest that 0.4% Cys supplementation is highly beneficial for maintaining the health of sows during late pregnancy. © 2019 The Royal Society of Chemistry.
语种：
英文
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Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate Citrobacter rodentium-Induced, Inflammation-Related Microbial and Metabolomic Profiles
作者：
Ma, Yong;Ding, Sujuan;Liu, Gang* ;Fang, Jun* ;Yen, Wenxin;...
期刊：
Frontiers in Microbiology ,2019年10(APR):643 ISSN：1664-302X
通讯作者：
Liu, Gang;Fang, Jun
作者机构：
[Liu, Gang; Yen, Wenxin; Ding, Sujuan; Liu, G; Fang, Jun; Ma, Yong; Jiang, Hongmei] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Liu, Gang] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha, Hunan, Peoples R China.;[Duraipandiyan, Veeramuthu; Esmail, Galal Ali; Al-Dhabi, Naif Abdullah] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh, Saudi Arabia.
通讯机构：
[Liu, G; Fang, J] H;[Liu, Gang] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, CAS Key Lab Agroecol Proc Subtrop Reg,Inst Subtro, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha, Hunan, Peoples R China.
关键词：
Citrobacter rodentium;IQW;IRW;inflammation;metabolomic;microbial
摘要：
Bioactive peptides that target the gastrointestinal tract can strongly affect the health of animals and humans. This study aimed to evaluate the abilities of two peptides derived from egg albumin transferrin, IRW and IQW, to treat enteritis in a mouse model of Citrobacter rodentium-induced colitis by evaluating serum metabolomics and gut microbes. Forty-eight mice were randomly assigned to six groups: basal diet (CTRL), intragastric administration Citrobacter rodentium (CR), basal diet with 0.03%IRW (IRW), CR with 0.03% IRW (IRW+CR), basal diet with 0.03%IQW (IQW) and CR with 0.03% IQW (IQW+CR). CR administration began on day 10 and continued for 7 days. After 14 days of IRW and IQW treatment, serum was collected and subjected to a metabolomics analysis. The length and weight of each colon were measured, and the colon contents were collected for 16srRNA sequencing. The colons were significantly longer in the CR group, compared to the CTRL group. A serum metabolomics analysis revealed no significant difference in microbial diversity between the six groups. Compared with the CTRL group, the proportions of Firmicutes and Actinobacteria species decreased significantly and the proportions of Bacteroidetes and Proteobacteria species increased in the CR group. There were no significant differences between the CTRL and other groups. The serum metabolomics analysis revealed that Infected by CR increased the levels of oxalic acid, homogentisic acid and prostaglandin but decreased the levels of L-glutamine, L-acetyl carnitine, 1-methylhistidine and gentisic acid. Therefore, treatment with IRW and IQW was shown to regulate the intestinal microorganisms associated with colonic inflammation and serum metabolite levels, thus improving intestinal health. Copyright © 2019 Ma, Ding, Liu, Fang, Yan, Duraipandiyan, Al-Dhabi, Esmail and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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英文
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不同高粱水平饲粮补充蛋白酶对生长猪的影响
作者：
李思思;方俊;李铁军
期刊：
湖南农业科学 ,2018年(4):70-74 ISSN：1006-060X
作者机构：
[李思思] 湖南农业大学生物科学技术学院,湖南 长沙 410128;[李思思] 中国科学院亚热带农业生态所亚热带农业生态过程重点实验室,湖南省畜禽健康养殖工程技术研究中心,农业部中南动物营养与饲料科学观测试验站,湖南 长沙 410125;湖南农业大学生物科学技术学院,湖南 长沙,410128;湖南畜禽安全生产协同创新中心,湖南 长沙 410128;[李铁军] 中国科学院亚热带农业生态研究所
关键词：
高粱;蛋白酶;生长猪;生长性能;肉品质;血清氨基酸
摘要：
为了研究不同高粱水平饲粮补充蛋白酶对生长猪生长性能、肉品质和血清氨基酸含量的影响,选取体重30±7 kg的生长猪设计了4个不同高粱添加水平(0％、20％、50％ 和80％)的饲养试验.结果显示:各组生长猪的平均日采食量、平均日增重和料重比均无显著差异(P>0.05);各组生长猪的肌肉pH45min值、pH24h值、L*值、a*值、b*值、剪切力等肉品质指标均无显著差异(P>0.05);用高粱替代玉米,并在饲粮中补充蛋白酶后,生长猪前腔静脉、肠系膜静脉和门静脉的血清组氨酸、甘氨酸、缬氨酸、丙氨酸、谷氨酸、丝氨酸、脯氨酸、甲硫氨酸、亮氨酸、酪氨酸含量有一定的变化.上述结果表明,用高粱替代20％～80％ 的玉米,并补充一定量的蛋白酶,可作为生长猪的饲粮.
语种：
中文
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