通讯机构：
[Yi-Ling Wen; Si Qin] K;[De-Xing Hou; Ziyu He] T;Key Laboratory for Food Science and Biotechnology of Hunan Province,College of Food Science and Technology,Hunan Agricultural University,China<&wdkj&>The United Graduate School of Agricultural Sciences,Faculty of Agriculture,Kagoshima University,Japan<&wdkj&>The United Graduate School of Agricultural Sciences,Faculty of Agriculture,Kagoshima University,Japan<&wdkj&>Key Laboratory for Food Science and Biotechnology of Hunan Province,College of Food Science and Technology,Hunan Agricultural University,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
Crocetin is a main bioactive component with a carotenoid skeleton in Gardenia jasminoides, a typical traditional Chinese medicine with a long history in Southeast Asia. Crocetin is being commonly consumed as spices, dyes, and food colorants. Recent pharmacological studies had implied that crocetin may possess potent anti-inflammatory properties; however, the underlying molecular mechanism is not fully elucidated. In the present study, the regulatory effect of crocetin on redox balance was systematically investigated in lipopolysaccharide- (LPS-) stimulated RAW264.7 cells. The results showed that crocetin dose-dependently inhibited LPS-induced nitric oxide production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 cells. Molecular data revealed that crocetin exerted its anti-inflammatory property by inhibiting the MEK1/JNK/NF-kappa B/iNOS pathway and activating the Nrf2/HO-1 pathway. The shRNA-knockdown (KD) of MEK1 and ERK1 confirmed that the activation of MEK1 and inhibition of JNK mediated the anti-inflammatory effect of crocetin. Moreover, the pull-down assay and computational molecule docking showed that crocetin could directly bind to MEK1 and JNK1/2. It is noticed that both KD and knockout (KO) of HO-1 gene blocked this action. More detailed data have shown that HO-1-KO blocked the inhibition of p-I kappa B-alpha by crocetin. These data indicated that crocetin exerted its anti-inflammatory property via modulating the crosstalk between the MEK1/JNK/NF-kappa B/iNOS pathway and the Nrf2/HO-1 pathway, highlighting HO-1 as a major player. Therefore, the present study reveals that crocetin can act as a potential candidate for redox-balancing modulation in charge of its anti-inflammatory and chemopreventive effect, which strengthens its potency in the subsequent clinic application in the near future.
期刊：
JOURNAL OF EXPERIMENTAL BOTANY,2021年72(4):1384-1398 ISSN：0022-0957
通讯作者：
Haoxuan Li<&wdkj&>Jianhua Zhang
作者机构：
[Wang, Guanqun; Li, Haoxuan; Zhang, Jianhua] Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China.;[Li, Xiaozheng] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen, Peoples R China.;[Li, Yongqiang; Li, Haoxuan; Zhang, Jianhua] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China.;[Li, Yongqiang; Li, Haoxuan; Zhang, Jianhua] Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China.;[Ye, Nenghui] Hunan Agr Univ, Coll Agr, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Peoples R China.
通讯机构：
[Haoxuan Li; Jianhua Zhang] D;Department of Biology, Hong Kong Baptist University , Kowloon, Hong Kong<&wdkj&>School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong , Shatin, Hong Kong
摘要：
Moderate soil drying (MD) imposed at the post-anthesis stage significantly improves carbon reserve remobilization in rice stems, increasing grain yield. However, the methylome and transcriptome profiles of carbon reserve remobilization under MD are obscure in indica and japonica rice stems. Here, we generated whole-genome single-base resolution maps of the DNA methylome in indica and japonica rice stems. DNA methylation levels were higher in indica than in japonica and positively correlated with genome size. MD treatment had a weak impact on the changes in methylation levels in indica. Moreover, the number of differentially methylated regions was much lower in indica, indicating the existence of cultivar-specific methylation patterns in response to MD during grain filling. The gene encoding β-glucosidase 1, involved in the starch degradation process, was hypomethylated and up-regulated in indica, resulting in improved starch to sucrose conversion under MD treatment. Additionally, increased expression of MYBS1 transactivated the expression of AMYC2/OsAMY2A in both indica and japonica, leading to enhanced starch degradation under MD. In contrast, down-regulated expression of MYB30 resulted in increased expression of BMY5 in both cultivars. Our findings decode the dynamics of DNA methylation in indica and japonica rice stems and propose candidate genes for improving carbon reserve remobilization.
作者机构：
[Zhong, Huan] Hunan Agr Univ, Utilizat Distinct Aquat Resource, Hunan Res Ctr Engn Technol, Changsha, Peoples R China.;[Zhou, Yi] Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Engn Res Ctr Polyploid Fish Reprod & Breeding, State Educ Minist,Coll Life Sci, Changsha, Peoples R China.;[Zhang, Hong] Beibu Gulf Univ, Beibu Gulf Marine Biodivers Conservat, Guangxi Key Lab, Qinzhou, Peoples R China.;[Xiao, Wei] Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resourc, Wuxi, Jiangsu, Peoples R China.
通讯机构：
[Yi Zhou] S;State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, China
通讯机构：
[Yu, Peng] H;Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.
关键词：
Lithium ion batteries;Anode materials;Silicon/carbon composites;Graphitized carbon;Magnesiothermic reduction
摘要：
Nanoporous silicon @ graphitized carbon (NPSi@C) composites have been reasonably designed and synthesized via one-step magnesiothermic co-reduction using rice husks as the silicon source and CO2 as the carbon source. In the self-assembly process, the nano-silicon prepared from rice husk-derived SiO2 is made into a porous structure and uniformly recombined with graphitized carbon formed by CO2 reduction at low temperature (680 degrees C). The nanoporous structure has contributed to accommodate the large volume effect of activated silicon and provides sufficient channels for the transfer of lithium ions. Furthermore, the graphitized carbon layers effectively enhance the electrical conductivity and coulombic efficiency of the composites, which is conducive to the improvement of electrochemical performance. The optimized NPSi@C composite exhibits superior lithium storage properties with the initial coulombic efficiency of 41.0% and the reversible specific capacity of 681.8 mA h g(-1) after 100 cycles at 0.2 A g(-1). This study suggests a simple, low-cost, and scalable strategy, which maximizes the advantages of traditional magnesiothermic reduction, to the preparation of Si/C composites as the most promising alternative anode materials for lithium ion batteries. (C) 2020 Elsevier B.V. All rights reserved.
通讯机构：
[Yao Li; Hongnan Liu] A;Authors to whom correspondence should be addressed.<&wdkj&>Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
摘要：
Simple Summary Oxidative stress and inflammation are common problems in livestock and poultry production, and have a serious impact on animal welfare and profitability. Finding an effective way to deal with oxidative stress and inflammation is an urgent problem for the modern pig industry. Cysteamine has been shown to play a crucial role in the growth performance, digestive metabolism, immune function, and fecundity of animals. In this study, we found that dietary supplementation of coated cysteamine could enhance the level of immunoglobulin and the expression of intestinal immune factors in the weaned pigs induced by diquat. It is proved that dietary supplementation with coated cysteamine can improve the intestine barrier function and immune function. This study aimed to explore the effects of dietary coated cysteamine on oxidative stress and inflammation in diquat-induced weaning pigs. Twenty-four pigs were randomly assigned to three dietary groups with eight replicates: the control (fed base diet), diquat (fed base diet), and coated cysteamine + diquat groups (fed 80 mg/kg cysteamine). The experiment was conducted for 21 d, and consisted of a pre-starter period (14 d) and a starter period (7 d). Coated cysteamine treatment significantly increased (p < 0.05) the final weight and average daily gain (ADG) in pigs. The contents of alkaline phosphatase (ALP), immunoglobulin G (IgG), serine (Ser), and isoleucine (Ile) were elevated (p < 0.05) while the contents of albumin (ALB) and aspartic acid (Asp) were reduced (p < 0.05) in the serum after coated cysteamine supplementation. Coated cysteamine supplementation resulted in greater (p < 0.05) serum superoxide dismutase (SOD) activity, the expression of interleukin-10 (IL-10) mRNA in the colon, and the CuSOD mRNA expression in the jejunum (p < 0.05) and colon (p = 0.073). Coated cysteamine supplementation showed an increasing trend in villus height (p = 0.060), villus height/crypt depth (V/C) (p = 0.056), the expression levels of zonula occludens-1 (ZO-1) mRNA (p = 0.061), and Occludin mRNA (p = 0.074) in the jejunum. In summary, dietary supplementation with coated cysteamine improves the intestinal barrier function of the jejunum by increasing the immunoglobulin content and the relative expression of intestinal immune factor mRNA in pigs while alleviating oxidative stress and inflammatory reactions caused by diquat.
通讯机构：
[Lin Tang] C;College of Environmental Science and Engineering, Hunan University, Changsha 410082, China<&wdkj&>Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, China
摘要：
Carbothermal reduction is a convenient and cost-effective method to produce biochar (BC) supported iron-based nano-particles (INP) for oxyanion contaminants removal. However, considering the possible desorption of the target oxyanion during change of the surrounding environment, the detailed removal mechanisms remain unclear and the long-term efficiency of different INPs cannot be predicted. In this study, different BC/Fe composites were synthesized by controlling the pyrolysis temperatures (500-800°C). BC/Fe(3)O(4) composite synthesized at 500°C (BC/Fe(500)) possessed the strongest surface acidity thus with the best SeO(4)(2-) removal performance, and BC/Fe(0)/Fe(3)O(4) composite synthesized at 650°C (BC/Fe(650)) possessed the best reducing ability toward SeO(4)(2-). Through the co-removal experiments (SeO(4)(2-) and common competing oxyanions co-existed) and the investigation of Se stability loaded on BC/Fe composites, the removal of SeO(4)(2-) by BC/Fe(500) through highly reversible adsorption could not achieve long-term immobilization of Se, making it an appropriate adsorbent for pre-treatment only, while the efficient reduction of SeO(4)(2-) to Se(0) by BC/Fe(650) could largely improve its long-term stability. This study supplies a possible strategy for Se immobilization against common competing oxyanions.
期刊：
Journal of Pharmacy and Pharmacology,2021年73(1):60-69 ISSN：0022-3573
通讯作者：
Aibing Wang
作者机构：
[Qin, Yan; Li, Bin; Peng, Caiyun; Wang, Wei; Qiu, Yixing; Yu, Huanghe; Guo, Shiyin; Liu, Leping; Jian, Yuqing] Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Academician Atta Ur Rahman Belt & Rd Tradit Med R, Sch Pharm, Changsha, Hunan, Peoples R China.;[Hu, Yi; Wang, Aibing; Qiu, Yixing; Lei, Xinnuo] Hunan Agr Univ, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, Changsha, Hunan, Peoples R China.
通讯机构：
[Aibing Wang] T;The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University , Changsha, Hunan, PR China
摘要：
Objectives To explore the in vivo anti-obesity effect of chikusetsusaponin V and explore the underlying mechanism by transcriptomic and metabonomic methods. Methods The physiological parameters of high-fat-diet induced obese mice administered with or without 25 mg/kg and 100 mg/kg of chikusetsusaponin V by gavage for 16 weeks were recorded. In addition, the RNA-sequencing and UHPLC-Q-TOF techniques were applied to obtain the transcriptomic and metabolomic profiling, respectively. Key findings Chikusetsusaponin V could significantly alleviate the high-fat-diet induced increase in the weight of the whole body and obesity-related organs or tissues, and ameliorate the lipid content in the blood, the lipid accumulation in the livers, as well as the hypertrophy of the fat tissues. Importantly, transcriptomic results revealed that more than 30 genes involved in the pathway which closely associates with obesity, were significantly altered. Moreover, metabolomic data indicated the key differential metabolites enriched in the pathways such as the activated protein kinase signaling pathway which is a vital mediator of obesity and other processes. Conclusions The integrative analysis highlighted that chikusetsusaponin V significantly influenced the activated protein kinase signaling pathway at both transcriptomic and metabolomic levels, thereby exerting anti-obesity effects.
通讯机构：
[Qing-Wen Gui; Qiang Li; Xiaoying Liu] C;College of Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128, People's Republic of China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha 410128, People's Republic of China<&wdkj&>College of Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128, People's Republic of China<&wdkj&>College of Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128, People's Republic of China<&wdkj&>State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China
摘要：
The electrochemical α-cyanation of tertiary and secondary amines has been developed by using a cheap cyanide reagent, azobisisobutyronitrile (AIBN). The CN radical, generated through n-Bu4NBr-meidated electrochemical oxidation, participates in a novel α-cyanation reaction under exogenous oxidant-free conditions.
摘要：
Our aim was to verify whether genes in the coagulation complement and cytokine interaction pathways are crucial to the resistance to grass carp hemorrhagic disease in grass carps (Ctenopharyngodon idella). To evaluate this, we acquired male grass carps with grass carp reovirus (GCRV) resistance, F1 generation, ordinary male fish, control F1 progenies (hybridized ordinary fish), and barbel chub (Squaliobarbus curriculus) as a positive control. We compared the expression variation of 13 immune factors in different tissues using real-time fluorescent quantitative PCR. Our results showed that the expression of the transforming growth factor-beta 3, nerve growth factor receptor, coagulation factor D, chemokine receptor XCR1, profibrinolysin, gamma-globulin of complement component 1, complement component 2, and complement component 5 in the different tissues of the barbel chub were significantly higher than those in the grass carps populations under normal conditions. Except for a limited number of genes, their expression exhibited significant differences. There was no significant difference in these genes among grass carp populations with different GCRV resistance. These results indicate that the high gene expression in the coagulation complement and cytokine interaction pathways may be the main reason for the strong resistance of barbel chub to GCRV. However, high gene expression was not a factor affecting GCRV resistance between grass carp populations.
摘要：
Liming materials have been extensively used to immobilize heavy metals. Bacteria, archaea, and fungi are sensitive to heavy metals and play critical roles in soil biogeochemical cycling and crop health. However, the responses of bacteria, archaea, and fungi to liming materials in agricultural soils contaminated with heavy metals are still unclear. A field experiment was conducted to assess the effect of liming on the three microbial domains and the potential influencing factors in maize-planted agricultural soil contaminated with Cd and Pb. Using a high-throughput sequencing method, we found that liming materials enhanced copiotrophic bacteria (Proteobacteria, Gemmatimonadetes, and Bacteroidetes), Bathyarchaeota, and arbuscular fungi (Glomeromycota) but suppressed some oligotrophic bacteria (Chloroflexi) and saprotrophic fungi. Notably, soil-available Cd markedly influenced the community composition of prokaryotes but not that of eukaryotes. Similar edaphic factors were correlated with the relative abundance of dominant bacterial and archaeal taxa but with opposite effects. Furthermore, co-occurrence network analysis revealed that the complexity decreased but the fungal nodes and edges increased after liming, indicating that the microbial community was not highly resilient to environmental stresses and the importance of fungi in community structure was enhanced. Overall, these findings suggest that changes in microorganisms resulting from liming facilitate soil biogeochemical cycles but suppress microbial stability, potentially affecting the sustainable use of agricultural soils contaminated with heavy metals.
通讯机构：
[Li, J.] H;[Chen, J.] A;Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, China;Animal Nutritional Genome and Germplasm Innovation Research Center, China
摘要：
Simple Summary Different breeds of pigs vary greatly in their propensity for adiposity. The gut microbiome plays a crucial role in shaping host physiological responses. However, it remains unclear how the gut microbiota influences host growth, in particular adipogenesis. This study aimed to compare microbial profiles in the colons of two pig breeds. Sixteen 35-day-old piglets, including eight Large White (LW) piglets (a lean-type pig breed) and eight Ningxiang (NX) piglets (a fatty-type Chinese Indigenous pig breed), were fed the same diet for 105 days. NX pigs had higher intramuscular fat content than LW pigs (p < 0.05). According to 16S rRNA gene sequencing, the relative abundances of the genera Ruminococcaceae_NK4A214_group, Parabacteroides, Christensenellaaceae_R-7_group and Ruminiclostridium were higher, whereas the abundances of Prevotellaceae_NK3B31_group, Prevotella, Subdoligranulum and Faecalibacterium were lower, in the colon of NX pigs compared to that of LW pigs. Nonmetric multidimensional scaling analysis revealed that the microbiota of the two pig breeds clustered separately along the principal coordinate axis. Furthermore, functional prediction of the bacterial communities suggested higher fatty acid biosynthesis in NX pigs. NX pigs also exhibited lower concentrations of total short-chain fatty acids, propionate and butyrate in the colon (p < 0.05). These findings suggest that NX pigs exhibited higher intramuscular fat content and backfat thickness than LW pigs. The bacterial communities in the colon of NX pigs were also more diverse than those in the colon of LW pigs, which might be used as a potential metabolomics mechanism to research different breeds of pigs.
通讯机构：
[Li, Y.; Huang, X.] C;[Li, Y.; Huang, X.] H;[Li, Y.; Huang, X.] T;Hunan Co-Innovation Center of Animal Production Safety, China;College of Animal Science and Technology, China
摘要：
The current study was performed to investigate whether dietary beta-hydroxy-beta-methylbutyrate (HMB) could regulate liver injury in a lipopolysaccharide- (LPS-) challenged piglet model and to determine the mechanisms involved. Thirty piglets (21 +/- 2 days old, 5.86 +/- 0.18 kg body weight) were randomly divided into the control (a basal diet, saline injection), LPS (a basal diet), or LPS+HMB (a basal diet + 0.60% HMB-Ca) group. After 15 d of treatment with LPS and/or HMB, blood and liver samples were obtained. The results showed that in LPS-injected piglets, HMB supplementation ameliorated liver histomorphological abnormalities induced by LPS challenge. Compared to the control group, the activities of serum aspartate aminotransferase and alkaline phosphatase were increased in the LPS-injected piglets (P<0.05). The LPS challenge also downregulated the mRNA expression of L-PFK, ACO, L-CPT-1, ICDH beta, and AMPK alpha 1/2 and upregulated the mRNA expression of PCNA, caspase 3, TNF-alpha, TLR4, MyD88, NOD1, and NF-kappa B p65 (P<0.05). However, these adverse effects of the LPS challenge were reversed by HMB supplementation (P<0.05). These results indicate that HMB may exert protective effects against LPS-induced liver injury, and the underlying mechanisms might involve the improvement of hepatic energy metabolism via regulating AMPK signaling pathway and the reduction of liver inflammation via modulating TLR4 and NOD signaling pathways.
通讯机构：
[Jinhai Yang] C;College of Business, Hunan University of Technology, Zhuzhou 412007, China
关键词：
Big data driven;Credit evaluation and early warning;Factor analysis;Internet of Things finance;Particle swarm optimization
摘要：
The big data technology framework has been successfully used in the Internet of Things, and the financial industry also hopes to use the advanced technology of big data to integrate and improve internal and external data related to credit risks. Relying on more efficient machine learning algorithms to get a reasonable prediction of credit risk can reduce the self-generated losses of the Internet of Things finance and increase profits. This article uses distributed search engine technology to customize web crawlers to obtain the required bank card and transaction data from the multi-source heterogeneous data of the Internet of Things financial industry, design the corresponding Spark parallel algorithm to preprocess the data, and establish an inverted table and two Level index file provides data source for big data analysis platform. After the data source is determined, the Mutually Exclusive Collectively Exhaustive (MECE) analysis method is combined with the scores of many financial business experts in the industry to obtain a set of candidate indicators and quantification methods for the financial credit risk evaluation of the Internet of Things, and analyze the correlation of indicators and risk grading. The random forest algorithm in the big data machine learning library is used to select the feature of the candidate index set, and a multi-level spatial association rule algorithm based on the Hash structure is designed to mine the financial risk information of the Internet of Things, and build a credit risk assessment and intelligent early warning model. This paper selects 26 indicators of Internet of Things finance as the research objects, uses SPSS26.0 software to perform sample Kaiser- Meyer-Olkin (KMO) test and Bartlett sphere test on the original data, and describes the results of factor analysis in detail. The particle swarm algorithm is introduced into the parameter optimization of random forest, and the financial credit risk assessment model of the Internet of Things is established. The results show that this method can significantly reduce the probability of banks making the first and second error rates when evaluating the credit risk of financing the Internet of Things Finance. This is conducive to the smooth development of the Internet of Things financial business for banks, which enables banks to enhance their own profitability while effectively reducing losses due to incorrect credit provision. (C) 2021 Elsevier B.V. All rights reserved.
作者机构：
[Lv, Ligang; Xiao, Tiaoyi; Liu, Yi; Xu, Baohong; Liu, Qiaolin; Su, Hang] Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.;[Ni, Jiajia] Guangdong Med Univ, Dongguan Key Lab Med Bioact Mol Dev & Translat Re, Dongguan 523808, Peoples R China.;[Ni, Jiajia] Guangdong Meilikang Biosci Ltd, Ctr Res & Dev, Dongguan 523808, Peoples R China.
通讯机构：
[Tiaoyi Xiao; Qiaolin Liu] H;Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, China
摘要：
Grass carp (Ctenopharyngodon idella) is the most widely produced freshwater aquaculture product worldwide. However, its susceptibility to microbial infections during its cultivation restricts its development. The complement system plays an important role in immune defence. To investigate the transcription of the complement factor C5 and production of its cleavage product C5a in grass carp infected with grass carp reovirus (GCRV), the full-length C5 gene sequence was cloned and sequenced using the rapid amplification of cDNA ends method, and C5 transcription and C5a production in different grass carp tissues at different GCRV infection stages were determined by RT-qPCR and western blotting, respectively. Our results showed that the full-length cDNA of the C5 gene was 5365-bp long, encoding 1687 amino acids. Signal peptide prediction showed that C5 had a secretory signal peptide. After artificial infection with GCRV, C5 transcription in grass carp liver increased significantly before the appearance of symptoms. C5a generation in the liver increased significantly from the onset of disease symptoms, and continued to increase until recovery. GCRV induced C5 cleavage in grass carp, with C5a production.
通讯机构：
[Cuiping Yi] S;School of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
whole-grain product;flat rice noodles;soy protein isolate;quality;flavor
期刊：
Science of The Total Environment,2021年752:141930 ISSN：0048-9697
通讯作者：
Yan, Xu
作者机构：
[Ke, Yong; Yan, Xu; Shi, Meiqing; Lin, Zhang; Peng, Ning; Min, Xiaobo; Yang, Zhihui; Wei, Yangjin; Wang, Sheng; Wu, Jiahui] Cent South Univ, Sch Met & Environm, Inst Environm Engn, Changsha 410083, Peoples R China.;[Ke, Yong; Yan, Xu; Shi, Meiqing; Lin, Zhang; Peng, Ning; Min, Xiaobo; Yang, Zhihui; Wei, Yangjin; Wang, Sheng; Wu, Jiahui] Chinese Natl Engn Res Ctr Control & Treatment Hea, Changsha 410083, Peoples R China.;[Yan, Xu] Water Pollut Control Technol Key Lab Hunan Prov, Changsha 410004, Peoples R China.;[Luo, Shuang] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.
通讯机构：
[Yan, Xu] C;Cent South Univ, Sch Met & Environm, Inst Environm Engn, Changsha 410083, Peoples R China.
关键词：
Heavy metals;Iron (oxyhydr)oxides;Retention mechanisms;Structural incorporation;Surface complexation;Surface precipitation
摘要：
Heavy metals are widespread toxic environmental pollutants that can generate enormous health and public concern. Iron (oxyhydr)oxides are ubiquitous in both natural and engineered environments and have great retention capacity of heavy metals due to their high surface areas and reactivity. The sequestration of heavy metal by iron (oxyhydr)oxides is one of the most vital geochemical/chemical processes controlling their environmental fate, transport, and bioavailability. In this review, some of the common iron (oxyhydr)oxides are introduced in detail in terms of their formation, occurrence, structure characteristics and interaction with heavy metals. Moreover, the retention mechanisms of metal cations (e.g., Pb, Cu, Cd, Ni, Zn), metal oxyanions (e.g., As, Sb, Cr), and coexisting multiple metals on various iron (oxyhydr)oxides are fully reviewed. Principal mechanisms of surface complexation, surface precipitation and structural incorporation are responsible for heavy metal retention on iron (oxyhydr)oxides, and greatly dependent on mineral species, metal ion species, reacting conditions (i.e., pH, heavy metal concentration, ionic strength, etc.) and chemical process (i.e., adsorption, coprecipitaton and mineral phase transformation process). The retention mechanisms summarized in this review would be helpful for remediating heavy metal contamination and predicting the long-term behavior of heavy metal in natural and engineered environments. (C) 2020 Elsevier B.V. All rights reserved.
