桦木酸对环磷酰胺致小鼠肝脏损伤的保护作用
作者：
马朝阳;朱利娟;罗晨曦;朱子寒;孔丽;...
期刊：
食品科学 ,2020年41(21):147-153 ISSN：1002-6630
作者机构：
湖南农业大学动物医学院, 湖南, 长沙, 410128;畜禽保健湖南省工程研究中心, 畜禽保健湖南省工程研究中心, 湖南, 长沙, 410128;湖南畜禽安全生产协同创新中心, 湖南畜禽安全生产协同创新中心, 湖南, 长沙, 410128;[罗晨曦; 易金娥; 马玉容; 林星; 高鑫禹; 朱子寒; 马朝阳; 孔丽; 欧朝萍; 朱利娟] 湖南农业大学
关键词：
桦木酸;环磷酰胺;氧化应激;抗炎;肝脏损伤
摘要：
为了探究桦木酸(betulinic acid,BA)对环磷酰胺(cyclophosphamide,CYP)导致的肝脏损伤的影响。将50只昆明小鼠随机分为5个组,即空白组、CYP组以及BA低、中、高剂量组。空白组和CYP组用1%(质量分数,下同)的可溶性淀粉灌胃,而其余各组按0.25、0.50、1.00 mg/kg m_b BA剂量混悬在1%可溶性淀粉糊中灌胃,连续灌胃14 d后,除空白组腹腔注射生理盐水外,其余各组连续2 d腹腔注射CYP(50 mg/kg m_b)诱导肝损伤模型。检测血清中谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)活力;检测肝脏超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)活力,以及还原型谷胱甘肽(glutathione, GSH)和丙二醛(malondialdehyde,MDA)含量;荧光定量聚合酶链式反应检测肝脏促炎因子白细胞介素(interleukin,IL)-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-1β和抗炎因子IL-10 mRNA的相对表达量。结果表明:1)与CYP组相比,BA预处理使小鼠体质量增加,其中0.50 mg/kg m_b BA组显著增加(P<0.05),同时使肝脏指数降低,其中0.50 mg/kg m_b和1.00 mg/kg m_b BA组显著降低(P<0.05);2)BA呈剂量依赖性降低AST的活力,其中1.00 mg/kg m_b BA组显著降低(P<0.05),0.50 mg/kg m_b BA组显著降低ALT活力(P<0.05);3)BA预处理缓解由CYP导致的空泡样变性;4)BA预处理降低肝脏SOD活力,其中0.25 mg/kg m_bBA组显著降低(P<0.05),BA对肝脏GSH-Px、CAT、GSH和MDA水平无明显影响(P>0.05); 5)0.25 mg/kg m_b和1.00 mg/kg m_b BA缓解CYP引起的IL-1β mRNA表达升高(P<0.05,P<0.01),BA极显著增加肝脏中IL-6(P<0.01)和IL-10 mRNA的表达(P<0.01),0.25 mg/kg m_b BA增加肝脏TNF-α mRNA的表达,但0.50 mg/kg m_b和1.00 mg/kg m_b BA降低肝脏TNF-α mRNA的表达(P<0.01)。结论:BA能抑制CYP引起的血清酶活力改变,改善小鼠肝脏的脂质过氧化,抑制促炎因子分泌,促进抗炎因子的分泌,对CYP诱导的肝脏损伤具有预防性保护作用。
语种：
中文
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Protective effects of betulinic acid on intestinal mucosal injury induced by cyclophosphamide in mice.
作者：
Wang, Xihong;Yuan, Zhihang;Zhu, Lijuan;Yi, Xianglian;Ou, Zhaoping;...
期刊：
Pharmacological Reports ,2019年71(5):929-939 ISSN：1734-1140
通讯作者：
Wu, Jing;Yi, Jine
作者机构：
[Tan, Zhuliang; Wu, Jing; Wang, Xihong; Ou, Zhaoping; Yi, Xianglian; Li, Rongfang; Zhu, Lijuan; Yi, Jine; Yuan, Zhihang; Yi, JN] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Pozniak, Blazej; Obminska-Mrukowicz, Bozena] Wroclaw Univ Environm & Life Sci, Fac Vet Med, Dept Pharmacol & Toxicol, Wroclaw, Poland.
通讯机构：
[Wu, J; Yi, JN] H;Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
关键词：
betulic acid;claudin 1;cyclophosphamide;glutathione;interleukin 10;interleukin 17;interleukin 1beta;interleukin 2;interleukin 5;macrophage inflammatory protein 1alpha;macrophage inflammatory protein 1beta;malonaldehyde;messenger RNA;protein ZO1;superoxide dismutase;tumor necrosis factor;antioxidant;betulic acid;cyclophosphamide;cytokine;nonsteroid antiinflammatory agent;triterpene;animal experiment;animal model;antioxidant activity;Article;controlled study;down regulation;drug mechanism;enzyme activity;enzyme inhibition;intestine injury;intestine mucosa;intestine villus;male;morphology;mouse;nonhuman;protein expression;regulatory mechanism;reverse transcription polymerase chain reaction;small intestine;treatment duration;animal;drug effect;immunology;inbred mouse strain;intestine mucosa;metabolism;oxidation reduction reaction;oxidative stress;pathology;Animals;Anti-Inflammatory Agents, Non-Steroidal;Antioxidants;Cyclophosphamide;Cytokines;Intestinal Mucosa;Intestine, Small;Male;Mice, Inbred Strains;Oxidation-Reduction;Oxidative Stress;Triterpenes
摘要：
Background: Betulinic acid (BA) is a plant-derived pentacyclic triterpenoid with a variety of biological activities. The purpose of this study was to assess the potential protective role of BA against intestinal mucosal injury induced by cyclophosphamide (CYP) treatment. Methods: Mice were pretreated with BA daily (0.05, 0.5, and 5.0 mg/kg) for 14 days, then injected intraperitoneally with CYP (50 mg/kg) for 2 days. Results: BA pretreatment reduced the contents of malondialdehyde (MDA) and glutathione (GSH), decreased the activity of superoxide dismutase (SOD) in small intestine, increased villus hight/crypt depth ratio and restored the morphology of intestinal villi in CYP-induced mice. Moreover, BA pretreatment could significantly down-regulate the levels of pro-inflammatory cytokines interleukin-5 (IL-5), IL-17, IL-12(P70) and tumor necrosis factor α (TNF-α), reduced production of chemokines macrophage inflammatory protein-1α (MIP-1α), macrophage inflammatory protein-1β (MIP-1β) and regulated upon activation, normal T-cell expressed and secreted (RANTES), and enhanced the levels of anti-inflammatory such as IL-2 and IL-10 in serum, and decreased the mRNA expressions of IL-1β and TNF-α in intestine of CYP-induced mice. Furthermore, RT-PCR demonstrated that BA improved intestinal physical and immunological barrier in CYP-stimulated mice by enhancing the mRNA expressions of zonula occluden 1 (ZO-1) and Claudin-1. Conclusions: BA might be considered as an effective agent in the amelioration of the intestinal mucosal resulting from CYP treatment. © 2019 Institute of Pharmacology, Polish Academy of Sciences
语种：
英文
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Anti-inflammatory effect and potential mechanism of betulinic acid on λ-carrageenan-induced paw edema in mice
作者：
Ou, Zhaoping;Zhao, Jing;Zhu, Lijuan;Huang, Lin;Ma, Yurong;...
期刊：
Biomedicine & Pharmacotherapy ,2019年118(118):109347 ISSN：0753-3322
通讯作者：
Li, Rongfang;Yi, Jine
作者机构：
[Luo, Chenxi; Ou, Zhaoping; Li, Rongfang; Yi, Jine; Ma, Chaoyang; Yuan, Zhihang; Zhu, Zihan; Wu, Jing; Li, RF; Yi, JN; Zhao, Jing; Zhu, Lijuan; Huang, Lin; Ma, Yurong] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Li, Rongfang; Yi, Jine; Yuan, Zhihang; Wu, Jing] Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Li, RF; Yi, JN] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Betulinic acid;Inflammation;lambda-Carrageenan;MAPK-COX-2-PGE(2) signaling pathway
摘要：
lambda-Carrageenan (Carr), a seaweed polysaccharide, is used as a proinflammatory agent in research. Betulinic acid (BA), a naturally occurring pentacyclic triterpenoid, exerts immunomodulatory, antioxidant, anti-inflammatory, anti-tumor, anti-malarial and anti-HIV effects. The aim of this study was to investigate whether BA exerts anti-inflammatory effect against Carr-induced paw edema in mice, and how BA could mediate the expression of inflammation-associated MAPK-COX-2-PGE(2) signal pathway. BA pretreatment significantly reduced the in-flammatory response to Carr-induced paw edema, especially at 4 h after injection. BA reduced the serum levels of pro-inflammatory cytokines, such as IL-1 alpha, IL-1 beta, IL-5, IL-6, GM-CSF, KC, MCP-1 and PGE(2) in Carr-treated mice, and increased those of anti-inflammatory cytokines, such as IL-12. It also increased SOD, CAT and GSH-Px activities, and GSH content, and reduced MDA content in the liver of Carr-treated mice. Besides, BA reduced neutrophil infiltration in the basal and subcutaneous layers of the paw of Carr-treated mice, decreased the expression of COX-2 protein, and reduced the phosphorylation of JNK, p38 and ERK1/2. These results indicated that the protective effect of BA on Carr-induced paw edema might be due to its alleviation of inflammatory response and inhibition of oxidative stress, possibly by inhibiting MAPK-COX-2-PGE(2) signaling pathway activation.
语种：
英文
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Ganoderma lucidum polysaccharides prevent palmitic acid-evoked apoptosis and autophagy in intestinal porcine epithelial cell line via restoration of mitochondrial function and regulation of MAPK and AMPK/Akt/mTOR signaling pathway
作者：
Liang, Zengenni;Yuan, Zhihang;Guo, Jiajing;Wu, Jing;Yi, Jine;...
期刊：
International Journal of Molecular Sciences ,2019年20(3):478 ISSN：1661-6596
通讯作者：
Shan, Yang
作者机构：
[Guo, Jiajing; Liang, Zengenni] Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Hunan, Peoples R China.;[Wu, Jing; Yi, Jine; Yuan, Zhihang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Deng, Jing; Shan, Yang] Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Shan, Yang] H;Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China.
关键词：
beclin 1;caspase 3;mammalian target of rapamycin;mitogen activated protein kinase;palmitic acid;polysaccharide;protein Bax;protein bcl 2;sequestosome 1;biological marker;fungal polysaccharide;palmitic acid;protein kinase B;target of rapamycin kinase;Akt/mTOR signaling;AMPK signaling;animal cell;animal tissue;anion exchange chromatography;apoptosis;Article;autophagosome;autophagy;bioluminescence;cell death;cell proliferation;cell protection;cell survival;cell viability;cell viability assay;Chinese medicine;controlled study;cytotoxicity;enzyme linked immunosorbent assay;flow cytometry;Ganoderma lucidum;human;human cell;IC50;immunofluorescence test;immunoreactivity;lipid diet;lipid peroxidation;MAPK signaling;mitochondrial membrane potential;mitophagy;mouse;MTT assay;nonhuman;oxidative stress;pig;protein expression;protein phosphorylation;signal transduction;upregulation;Western blotting;animal;Basidiomycetes;cell line;chemistry;drug effect;epithelium cell;intestine mucosa;metabolism;mitochondrion;phosphorylation;Animals;Apoptosis;Autophagy;Basidiomycota;Biomarkers;Cell Line;Epithelial Cells;Fungal Polysaccharides;Intestinal Mucosa;Mitochondria;Mitogen-Activated Protein Kinases;Palmitic Acid;Phosphorylation;Proto-Oncogene Proteins c-akt;Signal Transduction;Swine;TOR Serine-Threonine Kinases
摘要：
Ganoderma lucidum polysaccharide (GLP) extracted from Ganoderma lucidum (Leyss. ex Fr.) Karst, a traditional Chinese medicine, is a biologically active substance reported to possess anti-oxidative, anti-apoptotic, and neurological protection. However, it is unknown whether GLP have any protective effect against high-fat constituents-induced epithelial cell injury. The aim of this study was to investigate the protection and molecular mechanism of GLP on injury induced by palmitic acid (PA) in the intestinal porcine epithelial cell line (IPEC-J2). First, we tested whether the treatment of GLP attenuate PA-induced IPEC-J2 cell death. GLP markedly blocked PA-caused cytotoxicity and apoptosis in IPEC-J2 cells. Moreover, GLP recovered the decreased mitochondrial function and inhibited activation of caspase-dependent apoptotic pathway. Interestingly, PA promoted cell apoptosis and autophagy through stimulation of phosphorylation of mitogen-activated protein kinases (MAPKs), AMP-activated protein kinase (AMPK), and inhibition of phosphorylation of Akt and mammalian target of rapamycin (mTOR), which was reversed by GLP. Taken together, this study revealed a protective effect of GLP against PA-evoked IPEC-J2 cell death through anti-apoptotic and anti-autophagic properties. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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钩吻素子对H2O2诱导的IPEC-J2细胞氧化应激和凋亡的影响
作者：
李佳艳;邬静;易金娥;杨成林;王吉;...
期刊：
中兽医医药杂志 ,2019年38(3):5-8 ISSN：1000-6354
作者机构：
湖南农业大学动物医学院湖南省兽药工程技术研究中心,湖南长沙,410128;[王吉; 易金娥; 杨成林; 袁志航; 邬静; 李佳艳; 陈小军; 张明军] 湖南农业大学
关键词：
钩吻素子;IPEC-J2细胞;凋亡
摘要：
目的:探讨钩吻素子(koumine)对H2O2诱导的猪肠道上皮细胞(IPEC-J2)损伤的保护作用及其机制.方法:通过MTT法检测koumine对IPEC-J2细胞增殖的影响,并用H2O2诱导IPEC-J2细胞建立细胞损伤模型,通过MTT和LDH比色法检测分析koumine保护IPEC-J2细胞免受H2O2损伤的效果;生化方法检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性;蛋白免疫印迹(western blot)技术检测凋亡相关蛋白bax和bcl-2的表达情况;用流式细胞仪检测氧自由基(ROS)释放量和细胞凋亡率.结果:检测浓度范围内koumine对IPEC-J2细胞没有毒性;koumine可以通过调控IPEC-J2细胞中SOD活性、LDH漏出率、MDA水平和ROS的产生,保护H2O2对IPEC-J2细胞的氧化损伤,抑制H2O2引起的IPEC-J2细胞凋亡;koumine在抑制H2O2引起的细胞凋亡过程中,减少了促凋亡蛋白Bax表达,增加了抑凋亡蛋白Bcl-2表达,使得Bax/Bcl-2的比值在药物作用时随剂量的增加逐渐降低.结论:koumine能够保护IPEC-J2细胞对抗H2O2诱导的氧化损伤,具有一定的时间和剂量依赖关系,其作用机制与抑制细胞凋亡有关.
语种：
中文
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Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells
作者：
Wu, Jing;Zhou, Yu;Yuan, Zhihang;Yi, Jine;Chen, Jingshu;...
期刊：
TOXINS ,2019年11(1):45- ISSN：2072-6651
通讯作者：
Wang, Naidong;Tian, Yanan
作者机构：
[Wu, Jing; Wang, Naidong; Wang, ND; Tian, Yanan; Zhou, Yu; Yi, Jine; Yuan, Zhihang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wu, Jing; Yuan, Zhihang] Hunan Agr Univ, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Wu, Jing; Yuan, Zhihang] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Chen, Jingshu; Tian, Yanan] Texas A&M Univ, Coll Vet Med, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA.
通讯机构：
[Wang, ND; Tian, YA] H;[Tian, Yanan] T;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;Texas A&M Univ, Coll Vet Med, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA.
关键词：
T-2 toxin;toxicity;autophagy;apoptosis
摘要：
T-2 toxin is a mycotoxin generated by Fusarium species which has been shown to be highly toxic to human and animals. T-2 toxin induces apoptosis in various tissues/organs. Apoptosis and autophagy are two closely interconnected processes, which are important for maintaining physiological homeostasis as well as pathogenesis. Here, for the first time, we demonstrated that T-2 toxins induce autophagy in human liver cells (L02). We demonstrated that T-2 toxin induce acidic vesicular organelles formation, concomitant with the alterations in p62/SQSTM1 and LC3-phosphatidylethanolamine conjugate (LC3-II) and the enhancement of the autophagic flux. Using mRFP-GFP-LC3 by lentiviral transduction, we showed T-2 toxin-mediated lysosomal fusion and the formation of autophagosomes in L02 cells. The formation of autophagosomes was further confirmed by transmission electron microcopy. While T-2 toxin induced both autophagy and apoptosis, autophagy appears to be a leading event in the response to T-2 toxin treatment, reflecting its protective role in cells against cellular damage. Activating autophagy by rapamycin (RAPA) inhibited apoptosis, while suppressing autophagy by chloroquine greatly enhanced the T-2 toxin-induced apoptosis, suggesting the crosstalk between autophagy and apoptosis. Taken together, these results indicate that autophagy plays a role in protecting cells from T-2 toxin-induced apoptosis suggesting that autophagy may be manipulated for the alleviation of toxic responses induced by T-2 toxin.
语种：
英文
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Betulinic Acid Attenuates T-2-Toxin-Induced Testis Oxidative Damage Through Regulation of the JAK2/STAT3 Signaling Pathway in Mice
作者：
Wu, Jing;Yang, Chenglin;Liu, Juan;Chen, Jiaxin;Huang, Chao;...
期刊：
Biomolecules ,2019年9(12):787 ISSN：2218-273X
通讯作者：
Yi, Jin-E;Yuan, Zhihang
作者机构：
[Wu, Jing; Wang, Ji; Chen, Jiaxin; Yi, Jin-E; Liu, Juan; Yi, JE; Yuan, Zhihang; Yang, Chenglin; Huang, Chao; Wen, Lixin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;[Liang, Zengenni] Dept Hunan Agr Prod Proc Inst, Changsha 410128, Peoples R China.
通讯机构：
[Yi, JE; Yuan, ZH] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.
关键词：
JAK2/STAT3 signaling pathway;T-2 toxin;apoptosis;betulinic acid;oxidative stress;testis
摘要：
T-2 toxin is one of the most toxic type A trichothecene mycotoxins in nature, and it exhibits reproductive toxicity. Betulinic acid (BA) is a natural pentacyclic triterpene compound found in species of Betula, and it has been reported to have antioxidant activity. The aim of the present study was to investigate the protective effect of BA on T-2-toxin-induced testicular injury in mice and explore its molecular mechanism. Sixty adult male mice were randomly divided into groups. The mice were pretreated orally with BA (0.25, 0.5, and 1.0 mg/kg) daily for 14 days, and the T-2 toxin (4 mg/kg body weight) was administered via intraperitoneal injection to induce oxidative stress after the last administration of BA. BA pretreatment significantly increased the secreted levels of testosterone and sperm motility. Moreover, BA pretreatment significantly increased the total antioxidant capacity (T-AOC), the activity of SOD and CAT, and the content of GSH, and it reduced the content of MDA. Furthermore, BA relieved testicular injury and reduced the number of apoptotic cells, and it significantly decreased the protein expression of Janus kinase 2 (JAK2), signal transducers and activators of transcription 3 (STAT3), caspsae-3, and Bcl-2-associated X protein (Bax). BA also increased the expression of B-cell lymphoma-2 (Bcl-2). We suggest that BA reduced the oxidative damage induced by T-2 toxin, and that these protective effects may be partially mediated by the JAK2/STAT3 signaling pathway.
语种：
英文
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Phenethyl isothiocyanate induces IPEC-J2 cells cytotoxicity and apoptosis via S-G2/M phase arrest and mitochondria-mediated Bax/Bcl-2 pathway
作者：
Liu, Shuiping;Zhu, Yuanyuan;Yan, Sisi;Xiao, Haisi;Yi, Jine;...
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY ,2019年226:108574 ISSN：1532-0456
通讯作者：
Wu, Jing;Wen, Lixin
作者机构：
[Zhu, Yuanyuan; Wu, Jing; Liu, Shuiping; Li, Rongfang; Yi, Jine; Wen, Lixin; Xiao, Haisi; Yan, Sisi] Hunan Agr Univ, Coll Vet Med, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Wen, Lixin] Hunan Collaborat Innovat Ctr Anim Prod Safety, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Wu, Jing; Wen, Lixin] H;Hunan Agr Univ, Coll Vet Med, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;Hunan Collaborat Innovat Ctr Anim Prod Safety, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词：
Apoptosis;Cell cycle;IPEC-J2 cells;Mitochondria;Phenethyl isothiocyanate
摘要：
Phenethyl isothiocyanate (PEITC) is one of the glucosinolates (GLs) present in cruciferous vegetables. Although there are many reports of livestock and poultry poisoning caused by plants containing GLs, the actual dosage that causes poisoning and the characteristics of GLs and their metabolites are unclear. Herein, we investigated the inhibitory effects of PEITC on IPEC-J2 cells and examined the mechanisms of PEITC-induced apoptosis via the mitochondrial pathway. Cell viability was determined by the MTT assay, and the levels of reactive oxygen species, mitochondrial membrane potential (Psi), intracellular Ca(2+) concentration, and cell apoptosis were detected by flow cytometry. IPEC-J2 cells were collected to assess the activities of superoxide dismutase, catalase, and glutathione peroxidase, as well as the contents of glutathione, malondialdehyde, H2O2, ATP, and lactate dehydrogenase, using biochemical methods. The levels of cytochrome c, Bax, Bcl-2, caspase-3, caspase-9, poly (ADP-ribose) polymerase (PARP)-1, p53, CDC25C, and cyclin A2 were analyzed by western blotting. We found that PEITC effectively inhibited the growth of IPEC-J2 cells, causing apoptosis. PEITC suppressed the level of mitochondrial membrane potential; released cytochrome c from the mitochondria to the cytoplasm; reduced ATP levels; inhibited Bcl-2 expression; increased Bax expression; and activated caspase-9, caspase-3, and PARP-1, leading to apoptosis. PEITC also induced G2/M and S phase arrest by affecting cell cycle-associated proteins such as p53, CDC25C, and cyclin A2. We conclude that PEITC causes oxidative stress, cell cycle arrest, and apoptosis in IPEC-J2 cells via a mitochondrial-dependent Bax/Bcl-2 pathway.
语种：
英文
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Gamma-oryzanol prevents ethanol-induced liver injury by ameliorating oxidative stress and modulating apoptosis-related protein expression in mice
作者：
Zhu, Lijuan;Xiang, Siting;Ou, Zhaoping;Ma, Chaoyang;Yi, Xianglian;...
期刊：
Journal of Functional Foods ,2019年62:103532 ISSN：1756-4646
通讯作者：
Tan, Zhuliang;Yi, Jine
作者机构：
[Xiang, Siting; Ou, Zhaoping; Li, Rongfang; Wen, Lixin; Yi, Jine; Ma, Chaoyang; Tan, ZL; Yi, JN; Yuan, Zhihang; Wu, Jing; Tan, Zhuliang; Yi, Xianglian; Zhu, Lijuan; Huang, Lin; Ma, Yurong] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liang, Zengenni] Dept Hunan Agr Prod Proc Inst, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Tan, ZL; Yi, JN] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Gamma-oryzanol;Oxidative stress;Alcohol liver injury;Apoptosis
摘要：
Gamma-oryzanol (Orz), a mixture of ferulic acid esters of plant sterols and triterpene alcohols, has been proven to possess multiple activities. The purpose of this study was to explore the hepatoprotective effects and potential mechanisms of Orz against ethanol-induced liver injury in mice. Orz pretreatment significantly reduced the serum levels of ALP, ALT, AST and CYP2E1, and increased the TP and GLB levels in the ethanol-challenged mice. Orz increased the activities of CAT, SOD, GSH-PX and the GSH content in the liver, while decreased hepatic steatosis in ethanol-induced liver injure. Furthermore, Orz significantly decreased the protein expression of ASK1, JNK, P38, active-caspase-9 and active-caspase-3, and the protein ratio of Bax/Bcl-2 in livers of ethanol-challenged mice. These results suggest that Orz provides protection against ethanol-induced liver injury which might be due to its alleviation of oxidative stress and inhibition of apoptosis, possibly inhibiting MAPK signaling pathways mediated mitochondrial signaling pathway activation.
语种：
英文
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Protective effect of koumine, an alkaloid from gelsemium sempervirens, on injury induced by H 2 O 2 in IPEC-J2 cells
作者：
Yuan, Zhihang;Liang, Zengenni;Yi, Jine;Chen, Xiaojun;Li, Rongfang;...
期刊：
International Journal of Molecular Sciences ,2019年20(3):754 ISSN：1661-6596
通讯作者：
Wu, Jing;Sun, Zhiliang
作者机构：
[Wu, Yong; Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang; Wu, J] Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wu, Yong; Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang; Wu, J] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liang, Zengenni] Dept Hunan Agr Prod Proc Inst, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Wu, J; Sun, ZL] H;Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
关键词：
apoptosis regulatory protein;beta actin;caspase 3;caspase 9;catalase;glutathione;hydrogen peroxide;koumine;lactate dehydrogenase;malonaldehyde;matrix metalloproteinase;protein Bax;protein bcl 2;reactive oxygen metabolite;superoxide dismutase;trolox C;alkaloid;antioxidant;hydrogen peroxide;indole alkaloid;koumine;plant extract;reactive oxygen metabolite;animal cell;antioxidant activity;apoptosis;Article;bax bcl2 ratio;cell activity;cell damage;cell viability;chemical injury;controlled study;DNA damage;dose time effect relation;enzyme activation;enzyme activity;enzyme assay;enzyme release;flow cytometry;Gelsemium Sempervirens;histogram;IPEC-J2 cell line;metabolic ratio;mitochondrial injury;mitochondrial membrane potential;MTT assay;nonhuman;oxidative stress;protein expression;Western blotting;animal;apoptosis;cell line;cell survival;chemistry;drug effect;epithelium cell;Gelsemium;metabolism;oxidative stress;pig;Alkaloids;Animals;Antioxidants;Apoptosis;Cell Line;Cell Survival;Epithelial Cells;Gelsemium;Hydrogen Peroxide;Indole Alkaloids;Oxidative Stress;Plant Extracts;Reactive Oxygen Species;Swine
摘要：
Medicinal herbal plants have been commonly used for intervention in different diseases and improvement of health worldwide. Koumine, an alkaloid monomer found abundantly in Gelsemium plants, can be effectively used as an antioxidant. The purpose of this study was to evaluate the potential protective effect of koumine against hydrogen peroxide (H 2 O 2 )-induced oxidative stress and apoptosis in porcine intestinal epithelial cell line (IPEC-J2 cells). MTT assays showed that koumine significantly increased cell viability in H 2 O 2 -mediated IPEC-J2 cells. Preincubation with koumine ameliorated H 2 O 2 -medicated apoptosis by decreasing reactive oxygen species (ROS) production, and efficiently suppressed the lactate dehydrogenase (LDH) release and malondialdehyde (MDA) production. Moreover, a loss of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activities was restored to normal level in H 2 O 2 -induced IPEC-J2 cells upon koumine exposure. Furthermore, pretreatment with koumine suppressed H 2 O 2 -mediated loss of mitochondrial membrane potential, caspase-9 and caspase-3 activation, decrease of Bcl-2 expression and elevation of Bax expressions. Collectively, the results of this study indicated that koumine possesses the cytoprotective effects in IPEC-J2 cells during exposure to H 2 O 2 by suppressing production of ROS, inhibiting the caspase-3 activity and influencing the expression of Bax and Bcl-2. Koumine could potentially serve as a protective effect against H 2 O 2 -induced apoptosis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Koumine Promotes ROS Production to Suppress Hepatocellular Carcinoma Cell Proliferation Via NF-kappa B and ERK/p38 MAPK Signaling
作者：
Yuan, Zhihang;Liang, Zengenni;Yi, Jine;Chen, Xiaojun;Li, Rongfang;...
期刊：
Biomolecules ,2019年9(10):559 ISSN：2218-273X
通讯作者：
Wu, Jing;Sun, Zhiliang
作者机构：
[Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang] Hunan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Changsha 410128, Hunan, Peoples R China.;[Wu, Jing; Chen, Xiaojun; Li, Rongfang; Yi, Jine; Sun, Zhiliang; Yuan, Zhihang; Wu, J] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liang, Zengenni] Dept Hunan Agr Prod Proc Inst, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Wu, J; Sun, ZL] H;Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
关键词：
NF-kappaB and ERK/p38 MAPK signaling;ROS;hepatocellular carcinoma (HCC);koumine;proliferation
摘要：
In the past decades, hepatocellular carcinoma (HCC) has been receiving increased attention due to rising morbidity and mortality in both developing and developed countries. Koumine, one of the significant alkaloidal constituents of Gelsemium elegans Benth., has been regarded as a promising anti-inflammation, anxiolytic, and analgesic agent, as well as an anti-tumor agent. In the present study, we attempted to provide a novel mechanism by which koumine suppresses HCC cell proliferation. We demonstrated that koumine might suppress the proliferation of HCC cells and promote apoptosis in HCC cells dose-dependently. Under koumine treatment, the mitochondria membrane potential was significantly decreased while reactive oxygen species (ROS) production was increased in HCC cells; in the meantime, the phosphorylation of ERK, p38, p65, and IκBα could all be inhibited by koumine treatment dose-dependently. More importantly, the effects of koumine upon mitochondria membrane potential, ROS production, and the phosphorylation of ERK, p38, p65, and IκBα could be significantly reversed by ROS inhibitor, indicating that koumine affects HCC cell fate and ERK/p38 MAPK and NF-κB signaling activity through producing excess ROS. In conclusion, koumine could inhibit the proliferation of HCC cells and promote apoptosis in HCC cells; NF-κB and ERK/p38 MAPK pathways could contribute to koumine functions in a ROS-dependent manner. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines
作者：
Zhao, Jing;Li, Rongfang;Pawlak, Aleksandra;Henklewska, Marta;Sysak, Angelika;...
期刊：
IN VIVO ,2018年32(5):1081-1088 ISSN：0258-851X
通讯作者：
Yi, Jin-E;Obminska-Mrukowicz, Bozena
作者机构：
[Yi, Jin-E; Zhao, Jing; Li, Rongfang; Wen, Lixin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Pawlak, Aleksandra; Obminska-Mrukowicz, Bozena; Henklewska, Marta; Sysak, Angelika] Wroclaw Univ Environm & Life Sci, Dept Pharmacol & Toxicol, Fac Vet Med, CK Norwida 31, PL-50375 Wroclaw, Poland.
通讯机构：
[Yi, Jin-E] H;[Obminska-Mrukowicz, Bozena] W;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;Wroclaw Univ Environm & Life Sci, Dept Pharmacol & Toxicol, Fac Vet Med, CK Norwida 31, PL-50375 Wroclaw, Poland.
关键词：
Antiproliferative activity;Apoptosis;Betulin;Betulin acid;Canine lymphoma;Canine osteosarcoma;Cell cycle
摘要：
Background/Aim: Betulinic acid (BA) and betulin (BT) exhibit a variety of pharmacological properties including anti-cancer, anti-inflammatory and anti-oxidant ones. Canine lymphoma and osteosarcoma have a high mortality rate and need more effective therapeutic approaches. In this study, the anti-proliferative and pro-apoptotic effects of BA and BT were investigated in canine T-cell lymphoma (CL-1), canine B-cell lymphoma (CLBL-1) and canine osteosarcoma (D-17) cell lines. Materials and Methods: The cultured cells were treated with several concentrations of BA or BT for 24, 48 and 72 h, and cell proliferation was assessed by the MTT assay. Cell apoptotic rate and cell cycle were analyzed using flow cytometry. Results: Anti-proliferative effect of BT and BA was concentration- and time-dependent. Moreover, BA and BT arrested cell cycle in S phase in CL-1 and D-17 cells, and in G0/G1 phase in CLBL-1 cells. Conclusion: Both compounds showed an antitumor activity, and the effects of BA were stronger than that of BT. © 2018 Institute of Electrical and Electronics Engineers Inc. All rights reserved.
语种：
英文
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桦木酸对酒精性肝损伤小鼠线粒体凋亡通路的影响
作者：
马玉容;夏伟;易想炼;欧朝萍;袁志航;...
期刊：
营养学报 ,2018年40(6):599-603 ISSN：0512-7955
作者机构：
湖南农业大学动物医学院, 长沙, 410128;湖南省兽药工程技术技术研究中心, 湖南省兽药工程技术技术研究中心, 长沙, 410128;湖南畜禽安全生产协同创新中心, 湖南畜禽安全生产协同创新中心, 长沙, 410128;[马玉容; 夏伟; 易想炼; 欧朝萍] 湖南农业大学动物医学院, 长沙, 410128;[袁志航] 湖南农业大学动物医学院, 长沙, 410128 湖南省兽药工程技术技术研究中心, 湖南省兽药工程技术技术研究中心, 长沙, 410128
关键词：
桦木酸;酒精;肝损伤;线粒体凋亡通路
摘要：
目的研究桦木酸(betulinic acid,BA)对酒精性肝损伤小鼠线粒体信号通路的影响。方法56只健康雄性小鼠随机分为8组,即正常对照组(NC),酒精模型组(MC),BA低、中、高剂量组(L-BA,M-BA, H-BA,按0.25、 0.5、1 mg/kg bw给与),BA低、中、高剂量与酒精联合组(L-BAA, M-BAA, H-BAA) 。NC、MC组灌胃1%的可溶性淀粉,其余各组灌胃混悬于可溶性淀粉中不同剂量的BA,1/d,连续14d后,除NC组,L-BA, M-BA,H-BA组灌胃生理盐水外,其余4组均按10 ml/kg灌胃50%酒精诱发酒精性肝损伤模型。检测血清细胞色素P450 2E1(CYP2E1)水平,采用Western blot检测肝脏线粒体信号通路中Bcl-2、Bax、Caspase-9和Caspase-3蛋白表达的变化。结果BA预处理后,抑制由酒精引起的CYP2E1水平升高,显著降低酒精致肝损伤小鼠Bax/Bcl-2比值,明显减少Caspase-9和Caspase-3蛋白表达量,且呈量效关系。结论说明BA对酒精诱导肝损伤有预防性的保护作用,这种保护作用可能与调控线粒体凋亡通路有关。
语种：
中文
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板蓝根多糖对染铅雄性小鼠生长性能的影响
作者：
樊永恒;陈慧;邬静;易金娥;袁志航;...
期刊：
中兽医医药杂志 ,2018年37(3):60-63 ISSN：1000-6354
作者机构：
河南农业大学牧医工程学院,河南 郑州 450002;湖南农业大学动物医学院,湖南 长沙 410128;河南农业大学牧医工程学院,河南 郑州,450002;湖南农业大学动物医学院,湖南 长沙,410128;[易金娥; 袁慧; 袁志航; 邬静; 袁莉芸] 湖南农业大学
关键词：
醋酸铅;板蓝根多糖;雄性小鼠;生长性能
摘要：
研究板蓝根多糖(Radix Isatidis polysaccharide,RIPS)对醋酸铅诱导雄性小鼠生长发育的影响.将3周龄60只健康雄性昆明系小鼠随机分为6个组:即对照组,RIPS 100 mg/kg组,铅模型组,RIPS低、中、高剂量(50 mg/kg、100 mg/kg、 200 mg/kg)治疗组,连续7 d腹腔注射醋酸铅20 mg/kg制备小鼠铅中毒模型,造模后对板蓝根多糖治疗组分别饮水给予不同浓度的RIPS,1次/d,连续给药28 d.观测每组小鼠日均采食量、体质量增长、脏器系数.结果显示,模型组日均采食量少于对照组和RIPS组,RIPS治疗组日均采食量随RIPS剂量的增加而明显增加;染毒各组小鼠体质量均显著降低(P<0.01或P<0.05),100 mg/kg RIPS治疗组小鼠自给药第3周开始,体质量显著增加(P<0.05);模型组小鼠除肾脏指数显著降低于对照组(P<0.05),肝脏器指数虽有所增加,但无显著差异(P>0.05),50 mg/kg RIPS治疗组睾丸指数与100 mg/kg RIPS治疗组肝脏指数均显著低于模型组(P<0.05),100 mg/kg RIPS治疗组的肾脏指数则显著增加(P<0.05).提示板蓝根多糖对醋酸铅诱发的小鼠生长发育迟缓具有一定的保护作用,其作用机制有待后续研究.
语种：
中文
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桦木酸对环磷酰胺致小鼠肠道氧化损伤的影响
作者：
王喜红;朱利娟;易想炼;赵静;向思亭;...
期刊：
畜牧兽医学报 ,2018年49(4):818-824 ISSN：0366-6964
作者机构：
[王喜红; 朱利娟; 易想炼; 赵静; 向思亭; 谭柱良; 袁志航; 易金娥] 湖南农业大学动物医学院;[易金娥] 湖南畜禽安全生产协同创新中心
关键词：
桦木酸;氧化损伤;环磷酰胺;肠道黏膜
摘要：
旨在研究桦木酸(betulinic acid,BA)对环磷酰胺(cyclophosphamide,Cy)致小鼠肠道氧化损伤的影响。将健康雄性昆明小鼠随机分为5组:对照(NC)组、Cy组、0.5mg·kg~(-1) BA组、5.0mg·kg~(-1) BA组、50.0mg·kg~(-1) BA组。NC组和Cy组灌服1%的可溶性淀粉,其余各组按不同剂量的BA混悬于可溶性淀粉中灌服,1次·d~(-1),连续14d后,除NC组注射生理盐水外,其余4组均连续2d腹腔注射Cy(50mg·kg~(-1))诱导肠氧化损伤模型。检测肝、脾和胸腺指数,肠黏膜中分泌型免疫球蛋白α(secretory immunoglobulin a,SIgA)水平,血清和肠道中超氧化物歧化酶(superoxide dismutase,SOD)活性,还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde, MDA)含量变化。结果表明:BA预处理后,能缓解Cy引起的肝、脾和胸腺指数下降,促进肠黏膜SIgA的分泌,不同程度缓解了Cy引起小鼠血清和小肠MDA、GSH含量的升高,以中剂量效果最佳,BA对SOD活力无影响。结果表明BA能改善Cy引起的小鼠肠道脂质过氧化及提高肠道中SIgA的分泌,对Cy诱导的肠道氧化损伤有预防性的保护作用。
语种：
中文
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桦木酸对环磷酰胺致小鼠免疫器官氧化损伤的保护作用
作者：
易想炼;朱利娟;赵静;文利新;罗晨曦;...
期刊：
动物营养学报 ,2018年30(3):1199-1206 ISSN：1006-267X
作者机构：
[易想炼; 赵静; 罗晨曦; 黄淑贤; 邬静; 朱利娟; 文利新; 易金娥] 湖南农业大学动物医学院;[朱利娟] 长沙绿叶生物科技有限公司;[文利新; 易金娥] 湖南畜禽安全生产协同创新中心;[POZNIAK Bjazej] 弗罗茨瓦夫环境与生命科学大学兽医学院
关键词：
桦木酸;环磷酰胺;免疫器官;氧化应激
摘要：
为了研究桦木酸(BA)对环磷酰胺(Cy)诱导小鼠免疫器官氧化损伤的影响。将50只健康雄性昆明小鼠随机分为5组,即对照组、Cy组及0.05、0.50、5.00 mg / kg BW BA组。对照组和Cy组灌服1%的可溶性淀粉溶液,其余各组按不同剂量的BA灌服,连续14 d后,除对照组注射生理盐水外,其余4组均腹腔注射Cy(50 mg / kg BW)诱导氧化应激模型。收集血清、脾脏和胸腺,检测血清中谷丙转氨酶(ALT) 、谷草转氨酶(AST)活性及白蛋白(ALB) 、总蛋白(TP)含量,检测脾脏和胸腺超氧化物歧化酶(SOD) 、谷胱甘肽过氧化物酶(GSH-Px) 、过氧化氢酶(CAT)活性及还原型谷胱甘肽(GSH) 、丙二醛(MDA)含量。结果显示:1)对于体重,对照组与Cy组无显著差异(P>0.05),各剂量BA组与Cy组无显著差异(P>0.05) 。2)与对照组相比,Cy极显著提高了血清AST、ALT活性(P < 0.01),而5.00 mg / kg BW BA极显著缓解了这一现象(P<0.01) 。3)与对照组相比, Cy引起了脾脏指数和胸腺指数的显著降低(P < 0.05),而0.05 mg / kg BW BA显著缓解了胸腺指数的降低(P<0.05) 。4)与对照组相比,Cy组胸腺SOD活性、胸腺CAT活性、脾脏GSH含量显著或极显著下降(P<0.05或P<0.01),脾脏MDA含量极显著升高(P<0.01);与Cy组相比,0.50 mg / kg BW BA组显著或极显著提高了脾脏和胸腺GSH-Px和CAT活性及脾脏GSH含量(P <0.05或P <0.01),极显著降低脾脏MDA含量(P < 0.01),但极显著降低了胸腺SOD活性(P<0.01);与Cy组相比,5.00 mg / kg BW BA组显著或极显著提高了脾脏和胸腺GSH-Px和CAT活性(P<0.05或P<0.01),极显著降低脾脏MDA含量(P<0.01) 。由此可见,BA能够改善Cy引起的小鼠免疫器官的氧化应激,对Cy诱导的氧化损伤有预防性的保护作用。
语种：
中文
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Procyanidins B2 reverses the T-2 toxin-induced mitochondrial apoptosis in TM3 Leydig cells
作者：
Wn, Jing;Huang, Weimei;Xiao, Haisi;Xie, Yi;Yuan, Zhihang;...
期刊：
Journal of Functional Foods ,2018年45:118-128 ISSN：1756-4646
通讯作者：
Tu, Di;Tian, Yanan
作者机构：
[Tu, Di; Tian, Yanan; Yi, Jine; Wn, Jing; Huang, Weimei; Xiao, Haisi; Yuan, Zhihang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Chen, Jingshu; Tian, Yanan] Texas A&M Univ, Coll Vet Med, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA.;[Xie, Yi] Yiyang Vocat & Tech Coll, Yiyang 413000, Hunan, Peoples R China.;[Wn, Jing; Yuan, Zhihang] Hunan Agr Univ, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Wn, Jing; Yuan, Zhihang] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Tu, D; Tian, YN] H;Hunan Agr Univ HAU, Coll Vet Med, 1 Nongdalu Rd, Changsha 100071, Hunan, Peoples R China.
关键词：
Apoptosis;Oxidative stress;Procyanidins B2;T-2 toxin
摘要：
Procyanidins B2 (PB2) was found to block T-2 toxin-induced reproductive toxicity and may be used as a dietary preventive agent. We investigated the mechanism of PB2 in blocking the T-2 toxin-induced apoptosis in Leydig cells in conjunction with analysis of T-2 toxin induced proapoptotic responses in Leydig cells including induction of oxidative stresses. The stress inducible proteins ASK, p38 and JNK were increased in response to T-2 toxin and the induction was attenuated by PB2. Furthermore, we showed that T-2 toxin suppressed mitochondrial membrane potential, reduced the levels of the mitochondrial respiratory chain complex I-V, adversely affected the level of mitochondrial proteins CypD, Bax, Bak, Bcl-2, Bcl-xl, Bax/Bcl-2 and cyt c and these adverse effects were counteracted by PB2 in dose-dependent manner. In summary, our results showed great potential of PB2 in preventing T-2 toxin-induced toxicity in Leydig cells through its potent antioxidant property. © 2018
语种：
英文
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γ-谷维素对过氧化氢诱导L02细胞凋亡的保护作用
作者：
向思亭;易想炼;赵静;谭柱良;易金娥
期刊：
营养学报 ,2018年40(3):279-284 ISSN：0512-7955
作者机构：
湖南农业大学动物医学院, 长沙, 410128;湖南畜禽安全生产协同创新中心, 湖南畜禽安全生产协同创新中心, 长沙, 410128;[向思亭; 易想炼; 赵静; 谭柱良] 湖南农业大学动物医学院, 长沙, 410128;[易金娥] 湖南农业大学动物医学院, 长沙, 410128 湖南畜禽安全生产协同创新中心, 湖南畜禽安全生产协同创新中心, 长沙, 410128
关键词：
γ-谷维素;过氧化氢;细胞凋亡;L02细胞;DNA损伤
摘要：
目的探讨γ-谷维素（gamma-oryzanol,γ-Orz）对过氧化氢（hydrogen peroxide,O2）诱导人肝细胞（L02）凋亡的保护作用。方法 O2建立L02细胞凋亡模型,用不同浓度（0.1,0.2和0.4 mmol/L）γ-Orz对L02细胞进行预处理,检测细胞总抗氧化能力（total antioxidant capacity,T-AOC）与产生羟自由基（OH·）的能力,三磷酸腺苷酶（adenosine triphosphatase,ATPase）活力以及一氧化氮（nitric oxide,NO）水平;Hoechst 33258染色观察细胞形态变化;流式细胞术检测细胞周期变化;琼脂糖凝胶电泳观察细胞凋亡过程中DNA的裂解。结果γ-Orz呈剂量依赖性下调L02细胞产生NO水平,上调OH·的清除能力、ATP酶活力与T-AOC活性,调控各细胞周期的百分比,恢复细胞形态,有效改善O2诱导的细胞凋亡。结论γ-Orz对O2致L02细胞凋亡具有保护作用,与其抑制氧化应激,增强ATP酶活力,减少DNA损伤及细胞周期阻滞有关。
语种：
中文
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Betulinic acid attenuates dexamethasone-induced oxidative damage through the JNK-P38 MAPK signaling pathway in mice
作者：
Zhu, Lijuan;Yi, Xianglian;Zhao, Jing;Yuan, Zhihang;Wen, Lixin;...
期刊：
Biomedicine & Pharmacotherapy ,2018年103:499-508 ISSN：0753-3322
通讯作者：
Wu, Jing;Yi, Jine
作者机构：
[Tan, Zhuliang; Wu, Jing; Yi, J; Yi, Xianglian; Zhao, Jing; Zhu, Lijuan; Wen, Lixin; Yi, Jine; Yuan, Zhihang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Pozniak, Blazej; Obminska-Mrukowicz, Bozena] Wroclaw Univ Environm & Life Sci, Fac Vet Med, Dept Pharmacol & Toxicol, Norwida 31, PL-50375 Wroclaw, Poland.;[Zhu, Lijuan] Changsha Lvye Biotechnol Co Ltd, Changsha 410199, Hunan, Peoples R China.;[Tian, Yanan] Texas A&M Univ, Coll Vet Med, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA.
通讯机构：
[Wu, J; Yi, J] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Betulinic acid;Dexamethasone;Oxidative damage;Apoptosis;JNK;P38
摘要：
Dexamethasone (Dex), a potent anti-inflammatory/immunosuppressive agent, has been shown to induce oxidative stress. Betulinic acid (BA) is a pentacyclic lupane triterpene with a potent antioxidant activity. The aim of this study was to investigate the ameliorative effect and underlying mechanisms of BA on Dex-induced oxidative damage. Mice were pretreated with BA orally (0, 0.25, 0.5, and 1.0 mg/kg) daily for 14 days, and then a single dose of Dex (25 mg/kg body weight) was administered intraperitoneally 8 h after the last administration of BA to induce oxidative stress. BA pretreatment significantly alleviated Dex-induced changes of blood biochemical indices, increased the total antioxidant capacity (T-AOC), the activity of superoxide dismutase (SOD), and the ability of inhibiting hydroxyl radical (AIHR), reduced the level of malondialdehyde (MDA) in serum. Moreover, BA pretreatment enhanced the T-AOC, AIHR and the activity of peroxidase (POD) in liver, spleen and thymus. Concomitant with these biochemical parameters, BA pretreatment significantly reduced gene and protein expressions of apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK) and P38 mitogen-activated protein kinase (P38 MAPK) in the lymphatic organs of Dex-treated mice. BA was found to effectively attenuate Dex-induced oxidative damage. These protective effects may be mediated in part through the JNK-P38 MAPK signaling transduction pathway and BA may be a potential therapeutic agent due to its anti-oxidative properties.
语种：
英文
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桦木酸对地塞米松致小鼠氧化应激的机制研究
作者：
朱利娟;易想炼;赵静;王喜红;文利新;...
期刊：
动物营养学报 ,2018年30(3):1035-1043 ISSN：1006-267X
作者机构：
湖南农业大学动物医学院, 长沙, 410128;长沙绿叶生物科技有限公司, 长沙, 410125;弗罗兹瓦夫环境与生命科学大学兽医学院, 波兰, 弗罗兹瓦夫, 50-375;湖南畜禽安全生产协同创新中心, 湖南畜禽安全生产协同创新中心, 长沙, 410128;[朱利娟] 湖南农业大学动物医学院, 长沙, 410128 长沙绿叶生物科技有限公司, 长沙, 410125
关键词：
桦木酸;地塞米松;氧化应激;丝裂原活化蛋白激酶
摘要：
本试验旨在研究桦木酸(BA)对地塞米松(Dex)诱导氧化应激小鼠的保护作用和机制。将40只健康雄性昆明小鼠随机分为5组,即对照(NC)组、Dex组、0.25 mg / kg BA组、 0.50 mg / kg BA组、1.00 mg / kg BA组。NC组和Dex组小鼠灌服1 %的可溶性淀粉溶液,其余各组按不同剂量的BA灌服,连续14 d后,除对照组注射生理盐水外,其余4组均腹腔注射Dex(25 mg / kg BW)诱导氧化应激模型。检测各组小鼠肝脏、脾脏和胸腺总抗氧化能力(T-AOC) 、抑制羟自由基能力和过氧化物酶(POD)的活性,反转录(RT) -PCR检测脾脏和胸腺丝裂原活化蛋白激酶(MAPK)信号通路中凋亡信号调节激酶1(ASK1) 、c-Jun氨基末端激酶(JNK)和P38基因的表达量,蛋白质免疫印迹(Western Blot)法检测脾脏MAPK信号通路中ASK1、JNK和P38蛋白的表达量。结果表明:1)与NC组相比,Dex组的肝脏T-AOC、抑制羟自由基能力,脾脏POD活性,胸腺T-AOC、抑制羟自由基能力均极显著下降(P<0.01);与Dex组相比,0.50和1.00 mg / kg BA组肝脏T-AOC、抑制羟自由基能力以及POD活性均显著或极显著的升高(P < 0.05或P<0.01),0.50 mg / kg BA组脾脏T-AOC、0.50和1.00 mg / kg BA组脾脏抑制羟自由基能力、0.25和1.00 mg / kg BA组脾脏POD活性均显著或极显著的升高(P<0.05或P<0.01),0.50和1.00 mg / kg BA组胸腺T-AOC、抑制羟自由基能力和POD的活性均显著或极显著升高(P< 0.05或P<0.01) 。2)与NC组相比,Dex组脾脏和胸腺ASK1、JNK和P38 mRNA表达量均极显著升高(P < 0.01);与Dex组相比,0.50和1.00 mg / kg BA组脾脏和胸腺ASK1、JNK和P38 mRNA表达量均显著或极显著下降(P<0.05或P<0.01) 。3)与NC组相比,Dex组脾脏JNK和P38蛋白的表达量极显著升高(P<0.01);与Dex组相比,0.50 mg / kg BA组脾脏ASK1蛋白的表达量显著降低(P<0.05),0.25、0.50和1.00 mg / kg BA组脾脏JNK和P38蛋白的表达量显著或极显著降低(P<0.05或P<0.01) 。由此可见,BA预处理后,增强了Dex应激小鼠肝脏、脾脏和胸腺的T-AOC、抑制羟自由基能力和POD活性,降低了Dex应激小鼠脾脏和胸腺MAPK信号通路中ASK1、JNK和P38 mRNA表达量以及脾脏ASK1、JNK和P38蛋白的表达量。BA对Dex造成的氧化损伤具有预防性的保护作用,并且这种保护作用与JNK-P38 MAPK信号通路相关。
语种：
中文
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